April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Evaluation of Corneal Progenitor Cells in a Murine Model of Neurotrophic Keratopathy
Author Affiliations & Notes
  • Hiroki Ueno
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Giulio Ferrari
    Ophthalmology, Schepens Eye Res Inst/Harvard Univ, Boston, Massachusetts
  • Takaaki Hattori
    Ophthalmology, Schepens Eye Res Inst/Harvard Univ, Boston, Massachusetts
  • Satoki Ueno
    Ophthalmology, St Marianna Univ School of Med, Kawasaki, Japan
  • Sunil Chauhan
    Ophthalmology, Schepens Eye Res Inst/Harvard Univ, Boston, Massachusetts
  • Reza Dana
    MEEI/SERI Harvard Ophthalmology, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  Hiroki Ueno, None; Giulio Ferrari, None; Takaaki Hattori, None; Satoki Ueno, None; Sunil Chauhan, None; Reza Dana, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5143. doi:https://doi.org/
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      Hiroki Ueno, Giulio Ferrari, Takaaki Hattori, Satoki Ueno, Sunil Chauhan, Reza Dana; Evaluation of Corneal Progenitor Cells in a Murine Model of Neurotrophic Keratopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5143. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Neurotrophic keratopathy (NK) remains difficult to treat. It can cause corneal epithelial defects, stromal thinning and perforation suggesting a role of corneal nerves in maintaining the corneal homeostasis. The purpose of this study was to investigate the effect of corneal denervation on corneolimbal progenitor cells.

Methods: : NK was induced in mice by electro-coagulation of the trigeminal nerve and absence of corneal nerves was assessed by beta-III tubulin immunostaining. Real-time polymerase chain reaction was performed to quantify expression of corneal progenitor cell markers. ATP-binding cassette subfamily G member 2 (ABCG2), p63, hairy enhancer of split 1 (Hes1), Keratin 15 was assessed in denervated mice and controls by immunofluorescence microscopic studies. In addition, colony-forming efficiency (CFE) assay was performed to assess corneal progenitor cell function.

Results: : ABCG2, p63, Hes1 expression detected with immunostaining was decreased in denervated corneas after 7days compared to those in the normal corneas. Similarly, mRNA expression levels for ABCG2, p63, Hes1, N-cadherin and Keratin 15 were significantly decreased in denervated corneas. In addition, we found a significant reduction (~50%) in CFE of progenitor cells harvested from denervated eyes compared to normal eyes.

Conclusions: : Our results suggest that corneal progenitor cells are significantly reduced in number and function in our animal model of corneal denervation. Our data provide novel evidence for the critical role of innervation in maintaining corneal epithelial cells and/or the stem cell niche.

Keywords: cornea: epithelium • innervation: sensation • proliferation 
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