April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Limbal Fibroblast Conditioned Media: A Non-invasive Treatment for Limbal Stem Cell Deficiency
Behrad Y. Milani; Hossein Amirjamshidi; Hossein Sagha; Maryam Shafiq; Asadolah Movahedan; Robert M. Lavker; Beatrice Y. Yue; Ali R. Djalilian
Author Affiliations & Notes
  • Behrad Y. Milani
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Hossein Amirjamshidi
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Hossein Sagha
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Maryam Shafiq
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Asadolah Movahedan
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Robert M. Lavker
    Dermatology, Northwestern University Feinberg School of Medicine, Chicago, Illinois
  • Beatrice Y. Yue
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Ali R. Djalilian
    Ophthalmology and Visual Sciences, University Of Illinois at Chicago, Chicago, Illinois
  • Footnotes
    Commercial Relationships  Behrad Y. Milani, None; Hossein Amirjamshidi, None; Hossein Sagha, None; Maryam Shafiq, None; Asadolah Movahedan, None; Robert M. Lavker, None; Beatrice Y. Yue, None; Ali R. Djalilian, None
  • Footnotes
    Support  a Fight for Sight Grant-in-Aid, a career development grant K08EY017561-A1 to ARD, and a core grant EY01792 both from the National Institutes of Health, Bethesda, Maryland.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5144. doi:
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      Behrad Y. Milani, Hossein Amirjamshidi, Hossein Sagha, Maryam Shafiq, Asadolah Movahedan, Robert M. Lavker, Beatrice Y. Yue, Ali R. Djalilian; Limbal Fibroblast Conditioned Media: A Non-invasive Treatment for Limbal Stem Cell Deficiency. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5144.

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Abstract

Purpose: : Limbal fibroblasts are known to regulate the maintenance and differentiation of the corneal epithelium including the limbal epithelial stem cells. This study examined the effect of limbal fibroblast conditioned media in a mouse model of limbal stem cell deficiency.

Methods: : Limbal stem cell deficiency was created in C57/Bl6 mice by performing a limbus to limbus epithelial debridement. The mice were treated topically for 3 weeks with conditioned media derived from human limbal fibroblasts. The control mice were treated with skin fibroblast conditioned media or Dulbecco’s serum-free medium.

Results: : The mice treated with limbal fibroblast conditioned media demonstrated substantial growth of corneal type epithelial cells on the corneal surface with less conjunctival goblet cells. By contrast, the control treated corneas were found to be covered primarily by conjunctival type epithelium.

Conclusions: : Cell culture media conditioned by limbal fibroblasts appear to contain factor(s) that are therapeutically beneficial in a model of limbal stem cell deficiency. The current results further support the notion that the essential limbal stem cell niche is provided by limbal fibroblasts and suggest a new, non-invasive option in the treatment of limbal stem cell deficiency.

Keywords: cornea: epithelium • cornea: basic science • cornea: stroma and keratocytes 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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