April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Photobiomodulation Attenuates Retinal Degeneration in a Rodent Model of Retinitis Pigmentosa
Sandeep Gopalakrishnan; Betsy Abroe; Heather Schmitt; Adam M. Dubis; Phyllis Summerfelt; Joseph Carroll; Janis T. Eells
Author Affiliations & Notes
  • Sandeep Gopalakrishnan
    Health Sciences, University of Wisconsin, Milwaukee, Milwaukee, Wisconsin
  • Betsy Abroe
    Health Sciences, University of Wisconsin, Milwaukee, Milwaukee, Wisconsin
  • Heather Schmitt
    Health Sciences, University of Wisconsin, Milwaukee, Milwaukee, Wisconsin
  • Adam M. Dubis
    Cell Biology, Neurobiology and Anatomy,
    Medical College of Wisconsin, Milwaukee, Wisconsin
  • Phyllis Summerfelt
    Ophthalmology,
    Medical College of Wisconsin, Milwaukee, Wisconsin
  • Joseph Carroll
    Cell Biology, Neurobiology and Anatomy,
    Ophthalmology,
    Medical College of Wisconsin, Milwaukee, Wisconsin
  • Janis T. Eells
    Health Sciences, University of Wisconsin, Milwaukee, Milwaukee, Wisconsin
  • Footnotes
    Commercial Relationships  Sandeep Gopalakrishnan, None; Betsy Abroe, None; Heather Schmitt, None; Adam M. Dubis, None; Phyllis Summerfelt, None; Joseph Carroll, None; Janis T. Eells, None
  • Footnotes
    Support  JTE: FFB TA-NP-0709-0465-UWI, International Retinal Research Foundation. JC: NIH (EY017607, EY001931, EY014537), MCW Research Affairs Committee, RBP Career Development Award.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5465. doi:
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      Sandeep Gopalakrishnan, Betsy Abroe, Heather Schmitt, Adam M. Dubis, Phyllis Summerfelt, Joseph Carroll, Janis T. Eells; Photobiomodulation Attenuates Retinal Degeneration in a Rodent Model of Retinitis Pigmentosa. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5465.

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Abstract

Purpose: : Irradiation by light in the far-red to NIR region of the spectrum (photobiomodualtion, PBM) has been demonstrated to attenuate the severity of neurodegenerative disease in experimental and clinical studies. The purpose of this study was to test the hypothesis that a brief course of PBM would protect against the loss of retinal function and improve photoreceptor survival in a rodent model of retinitis pigmentosa.

Methods: : P23H-1 pups were treated once per day for 180 seconds with 830 nm light (25 mW/cm2; fluence 4 J/cm2) using a light-emitting diode array (Quantum Devices, Barneveld WI) from postnatal day p10 to p25. Sham-treated rats were restrained for 180 seconds, but not exposed to NIR light. The status of the retina was determined at p30 by measuring indices of photoreceptor function by ERG (scotopic series from 100mcd.s/m2 to 25000mcd.s/m2) and retinal morphology by Spectral Domain Optical Coherence Tomography (SD-OCT; Bioptigen, Inc).

Results: : 830 nm PBM protected against retinal degeneration in the P23H rat as assessed by ERG and SD-OCT. In NIR treated animals the a-wave amplitude at 1000 mcd.s/m2 was 117 ± 10 µV compared to 77 ± 7 µV in sham treated animals (p=0.007). The b-wave amplitude in NIR treated rats was 643 ± 49 µV compared to 415 ± 27 µV in sham treated animals (p=0.002). Total retinal thickness measured by SD-OCT linear scans was 179 ± 8 µm in NIR treated animals compared to 147± 8 µm for sham treated animals (p=0.04).

Conclusions: : Studies from our laboratory have documented preservation of retinal function and attenuation of photoreceptor loss by PBM in animal models of mitochondrial injury and light-induced retinal degeneration. Results from this study demonstrate the retinoprotective effects of 830nm PBM in a transgenic animal model of retinitis pigmentosa and support the use of PBM as an innovative, non-invasive therapeutic approach for the treatment of retinal degenerative disease.

Keywords: retinal degenerations: hereditary • photoreceptors • mitochondria 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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