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Vittorio Porciatti, Tsung-Han Chou; Adaptive Changes In The Pattern Electroretinogram (PERG) Induced By Flickering Light In The Mouse. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5477.
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© ARVO (1962-2015); The Authors (2016-present)
Light flickering at ~10 Hz induces dilation of retinal vessels, implying adaptation to increased metabolic demand. We tested the hypothesis that the PERG, a measure of retinal ganglion cell function, would be altered by superimposition of flicker in the mouse, and that PERG alteration would be relatively greater in DBA/2J mice prone to glaucoma with potentially altered baseline metabolism.
PERGs were recorded in 32 mice 2-3 months old (C57BL/6J, n=9; DBA/2J, n=10; DBA/2J.Gpnmb+, n=13) with superimposed flicker at either 10 Hz (test) or 100 Hz (control - beyond temporal resolution of mouse cone ERG). Flicker-induced PERG amplitude/latency changes were calculated as differences between test and control. PERG stimuli were horizontal gratings of 0.05 cycles/deg and 100% contrast. Flicker stimuli were bright white LED, and were asynchronous with the PERG stimulus, in order not to generate any recordable flicker ERG.
Baseline PERG amplitudes were similar in the three strains. Superimposition of 10 Hz flicker induced increases of PERG latency in all strains (C57BL/6J, 15.4 ms (SE 2.7); DBA/2J, 22.3 ms (SE 3.8); DBA/2J.Gpnmb+, 5.9 ms (SE 3.1). Strain differences in latency were significant (ANOVA, P=0.006). Flicker-induced amplitude changes were unremarkable and not different among strains (ANOVA, P=0.25).
Previous studies in the rat have shown increased arterial blood flow and PO2 during flicker at ~10 Hz. Our results indicate that these metabolic changes are associated with increased PERG latency at constant PERG amplitude. PERG latency changes are relatively greater in pre-glaucomatous DBA/2J mice, possibly indicating altered neurovascular/neurometabolic response in a strain prone to glaucoma.
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