April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Ultrastructure of Lens Capsule for Experimental Endophthalmitis
Author Affiliations & Notes
  • Yuka Suzuki
    Ophthalmology, Toho University Omori hospital, Ota-ku, Japan
  • Shinichiro Kobayakawa
    Ophthalmology, Toho University Omori hospital, Ota-ku, Japan
  • Kumiko Tanaka
    Ophthalmology, Toho University Omori hospital, Ota-ku, Japan
  • Tetuso Tochikubo
    Ophthalmology, Toho University Omori hospital, Ota-ku, Japan
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5592. doi:
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      Yuka Suzuki, Shinichiro Kobayakawa, Kumiko Tanaka, Tetuso Tochikubo; The Ultrastructure of Lens Capsule for Experimental Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5592.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the ultrastructure of lens capsule for experimental endophthalmitis in removed porcine eyes.

Methods: : Staphylococcus epidermidis, ATCC 35984 (biofilm-producer) and methicillin-resistant Staphylococcus aureus (MRSA), UA-1 (clinically isolated strain) were used. Small quantities of the culture were subcultured on the BHI broth overnight at 37°C. Then, cultures were kept frozen at -75°C in MicrobankTM (PRO-LAB Diagnostics, Toronto, Canada ). Phacoemulsification were performed in fresh removed porcine eyes, the incision size was increased to approximately 5-6 mm. Two beads from MicrobankTM were inserted into the capsular bag. Wound closure was achieved with a 8-0 Vicryl suture, 0.1-0.2 ml of the BHI broth was administered in the anterior chamber of the surgical pig eye at the end of surgery.Those porcine eyes were kept in a sterile 50ml plastic centrifuge tubes with 10ml corneal storage media (EP2, Kaken Pharmaceutical Co., Ltd., Tokyo, Japan) at 37°C after surgery. After incubation for 24 hours, those eyes were fixed with 2% glutaraldehyde for scanning electron microscopy (SEM).The bacteria on the surface of lens capsule were examined by SEM. The bacterial population at posterior capsule surface was also enumerated.

Results: : As for the S.epidermidis, those bacteria aggregated on the surface of lens capsule, while MRSA infiltrated to the lens capsule. Rates of bacteria-positive SEM fields were 10% (control), 37% (S.epidermidis) and 78% (MRSA). Moreover, rates of dozens bacteria-positive SEM fields were 16% (S.epidermidis) and 47% (MRSA).

Conclusions: : Bacteria may spread to the vitreous cavity and anterior chamber passing through the lens capsule in experimental endophthalmitis. Permeability of capsule membrane may be depending on bacterial species.

Keywords: endophthalmitis • anatomy • microbial pathogenesis: clinical studies 
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