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Yuka Suzuki, Shinichiro Kobayakawa, Kumiko Tanaka, Tetuso Tochikubo; The Ultrastructure of Lens Capsule for Experimental Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5592.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the ultrastructure of lens capsule for experimental endophthalmitis in removed porcine eyes.
Staphylococcus epidermidis, ATCC 35984 (biofilm-producer) and methicillin-resistant Staphylococcus aureus (MRSA), UA-1 (clinically isolated strain) were used. Small quantities of the culture were subcultured on the BHI broth overnight at 37°C. Then, cultures were kept frozen at -75°C in MicrobankTM (PRO-LAB Diagnostics, Toronto, Canada ). Phacoemulsification were performed in fresh removed porcine eyes, the incision size was increased to approximately 5-6 mm. Two beads from MicrobankTM were inserted into the capsular bag. Wound closure was achieved with a 8-0 Vicryl suture, 0.1-0.2 ml of the BHI broth was administered in the anterior chamber of the surgical pig eye at the end of surgery.Those porcine eyes were kept in a sterile 50ml plastic centrifuge tubes with 10ml corneal storage media (EP2, Kaken Pharmaceutical Co., Ltd., Tokyo, Japan) at 37°C after surgery. After incubation for 24 hours, those eyes were fixed with 2% glutaraldehyde for scanning electron microscopy (SEM).The bacteria on the surface of lens capsule were examined by SEM. The bacterial population at posterior capsule surface was also enumerated.
As for the S.epidermidis, those bacteria aggregated on the surface of lens capsule, while MRSA infiltrated to the lens capsule. Rates of bacteria-positive SEM fields were 10% (control), 37% (S.epidermidis) and 78% (MRSA). Moreover, rates of dozens bacteria-positive SEM fields were 16% (S.epidermidis) and 47% (MRSA).
Bacteria may spread to the vitreous cavity and anterior chamber passing through the lens capsule in experimental endophthalmitis. Permeability of capsule membrane may be depending on bacterial species.
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