April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Evaluation Of The Retinal Bioavailability After Parenteral Administration Of A DHEA Synthetic Analogue In The Rat Retina
Author Affiliations & Notes
  • Chrysanthi Tsika
    Department of Neurology and Sense Organs,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Pavlina A. Tsoka
    Department of Neurology and Sense Organs,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Manolis Tzatzarakis
    Department of Forensic Sciences and Toxicology,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Ioannis Charalampopoulos
    Department of Pharmacology,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Achilleas Gravanis
    Department of Pharmacology,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Miltiadis K. Tsilimbaris
    Department of Neurology and Sense Organs,
    Faculty of Medicine, University of Crete, Heraklion, Greece
  • Footnotes
    Commercial Relationships  Chrysanthi Tsika, None; Pavlina A. Tsoka, None; Manolis Tzatzarakis, None; Ioannis Charalampopoulos, None; Achilleas Gravanis, None; Miltiadis K. Tsilimbaris, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5634. doi:
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      Chrysanthi Tsika, Pavlina A. Tsoka, Manolis Tzatzarakis, Ioannis Charalampopoulos, Achilleas Gravanis, Miltiadis K. Tsilimbaris; Evaluation Of The Retinal Bioavailability After Parenteral Administration Of A DHEA Synthetic Analogue In The Rat Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5634.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the retinal bioavailability of a synthetic Dehydroepiandrosterone (DHEA) analogue, a molecule with potential anti-apoptotic action, after systemic administration.

Methods: : 8 Sprague Dawley rats were injected intraperitoneally with 10 mg of the analogue in1 ml of an ethanol/Water For Injection (WFI) solution. The synthetic neurosteroid was injected in 2 rats at each time point at the same concentration every time. The animals were euthanized at 15, 30, 60 and 120 min. The eyes were enucleated and the retina was isolated from the eye cup with an eye sponge with suitable handling. After appropriate preparation, the samples were measured with HPLC LC/MS.

Results: : The recovery of the method was 82.9%. The Limit Of Quantification (LOQ) was 5ng/ml. The substance was detected at 60 and 120 min in mean concentrations of 46.9 ng/ml and 183.8 ng/ml respectively. At the time points 15 and 30 no substance was detected with this method. No substance was detected in the blind samples.

Conclusions: : The synthetic DHEA analogue was successfully detected in the rat retina with an LC/MS HPLC method. The molecule seems to reach the retina in approximately one hour after systemic administration and is still detected there after two hours. Further investigation is obligatory for complete data of the pharmacokinetics of the substance in the rat retina.

Keywords: drug toxicity/drug effects • retina 
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