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Rachel Ka Man Chun, Dennis Y.Y. Tse, Bing Zuo, K.K. Li, G. Zhang, Q. Liu, S.A. McFadden, C.H. To; Proteome Analysis Of Guinea Pig Retina During Recovery From Lens-induced Myopia. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5646.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the differential protein expressions in pigmented guinea pigs during recovery from lens-induced myopia
Pigmented guinea pigs at the age of 4 days after birth were raised with -5D lens worn over right eyes and plano lens over left eyes for 2 weeks. Refractive errors and ocular parameters were measured using retinoscopy and A-scan ultrasound respectively. After that, the -5D lenses were removed from the right eyes for 48 hours. Refractive errors and ocular parameters were measured again after recovery. Retinas were extracted and homogenized in lysis buffer. Lysed retinas were labeled using CyDye Fluor minimal dyes. The retinal proteins were then separated under isoelectric focusing and second dimension of electrophoresis. Differential protein expressions between recovery and control retinas were analyzed using DeCyder Differential Analysis Software. Those differential proteins were identified by nano LC-MS/MS.
After 2 weeks of lens wear, all the right eyes became relatively myopic compared to fellow eyes. Mean interocular differences in refractive errors were -2.4D ± 0.70 (Mean ± SEM, n=4). The treated eyes showed a longer axial length (Mean interocular difference ± SEM, 51µm ± 13). After removal of -5D lenses for 48 hours, the interocular differences in refractive errors showed a significant hyperopic shift (Mean ± SEM, -0.83D ± 0.48, p<0.01, paired t-test). Smaller interocular difference in axial length was also found after recovery (Mean ± SEM, 32µm ± 12). Analysis of retinal proteome revealed five up-regulated and four down-regulated proteins after recovery. Five of them were successfully identified by LC-MS/MS. Two up-regulated proteins belonged to the same identity, alpha enolase. The others were transaldolase, phosphoglycomutase and a hypothetical protein.
Several proteins involved in glycolysis were found to be altered in mammalian retinas during recovery phase. It suggested that glycolytic rate is slower during the recovery phase when the refractive errors and axial length elongation are going back to normal. Conversely, it shows that myopia is indeed a process of accelerated eye growth which involves an increase of metabolism in terms of the glycolytic pathway.
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