April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Interfacing Biomaterials With The Retina In Vivo
Author Affiliations & Notes
  • Adrian P. Rowley
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Rodrigo Brant
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Changhong Zhang
    Chemistry,
    USC, Los Angeles, California
  • Mark Thompson
    Chemistry,
    USC, Los Angeles, California
  • Steve Swenson
    Biochemistry and Molecular Biology,
    USC, Los Angeles, California
  • Frank Markland
    Biochemistry and Molecular Biology,
    USC, Los Angeles, California
  • James D. Weiland
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Mark S. Humayun
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Footnotes
    Commercial Relationships  Adrian P. Rowley, P (P); Rodrigo Brant, None; Changhong Zhang, None; Mark Thompson, None; Steve Swenson, None; Frank Markland, None; James D. Weiland, None; Mark S. Humayun, None
  • Footnotes
    Support  Department of Energy's Office of Science; Grant Number: DE-FC02-04ER63735
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5653. doi:
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    • Get Citation

      Adrian P. Rowley, Rodrigo Brant, Changhong Zhang, Mark Thompson, Steve Swenson, Frank Markland, James D. Weiland, Mark S. Humayun; Interfacing Biomaterials With The Retina In Vivo. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5653.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To study the in vivo effects of polymers coated with a peptide, Vicrostatin (VCN), and temperature responsive plasma polymerized N-isopropyl acrylamide (pNIPAM) for adhesion to retinal tissue, in rabbit eyes.

Methods: : Parylene C (poly(monochloro-p-xylylene)) (30 µm) coated with VCN and pNIPAM were used as implant materials. Following pars plana vitrectomy (PPV), VCN coated parylene (n=6) and pNIPAM coated parylene (n=6) implants were attached to the retina, in twelve rabbits. Baseline and follow-up imaging (color fundus photographs, fluorescein angiography, and optic coherence tomography [OCT]) were performed. Histological evaluation was performed following enucleation at 1, 2 and 3 months.

Results: : Intraoperative retinal adhesion occurred in all eyes with VCN and pNIPAM coated parylene implants. VCN (n=4) and pNIPAM (n=3) implants remained attached to the retinal surface with minimal macroscopic change. However, VCN (n=2) and pNIPAM (n=3) implants developed complications such as retinal detachment or retinal tears, usually during the implantation procedure. OCT findings confirmed retinal adhesion in all eyes (n=12) prior to enucleation. Histology showed mild changes at the outer retinal segments to full thickness tears. There was no evidence of ocular toxicity, although a number of eyes developed epiretinal membranes and some inflammatory changes at the interface.

Conclusions: : VCN and pNIPAM coated implants may provide retinal adhesion in vivo for use in medical devices. Improved surgical techniques should enable a reduction in the complication rate.

Keywords: retina • vitreoretinal surgery • cell adhesions/cell junctions 
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