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Adrian P. Rowley, Rodrigo Brant, Changhong Zhang, Mark Thompson, Steve Swenson, Frank Markland, James D. Weiland, Mark S. Humayun; Interfacing Biomaterials With The Retina In Vivo. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5653. doi: https://doi.org/.
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To study the in vivo effects of polymers coated with a peptide, Vicrostatin (VCN), and temperature responsive plasma polymerized N-isopropyl acrylamide (pNIPAM) for adhesion to retinal tissue, in rabbit eyes.
Parylene C (poly(monochloro-p-xylylene)) (30 µm) coated with VCN and pNIPAM were used as implant materials. Following pars plana vitrectomy (PPV), VCN coated parylene (n=6) and pNIPAM coated parylene (n=6) implants were attached to the retina, in twelve rabbits. Baseline and follow-up imaging (color fundus photographs, fluorescein angiography, and optic coherence tomography [OCT]) were performed. Histological evaluation was performed following enucleation at 1, 2 and 3 months.
Intraoperative retinal adhesion occurred in all eyes with VCN and pNIPAM coated parylene implants. VCN (n=4) and pNIPAM (n=3) implants remained attached to the retinal surface with minimal macroscopic change. However, VCN (n=2) and pNIPAM (n=3) implants developed complications such as retinal detachment or retinal tears, usually during the implantation procedure. OCT findings confirmed retinal adhesion in all eyes (n=12) prior to enucleation. Histology showed mild changes at the outer retinal segments to full thickness tears. There was no evidence of ocular toxicity, although a number of eyes developed epiretinal membranes and some inflammatory changes at the interface.
VCN and pNIPAM coated implants may provide retinal adhesion in vivo for use in medical devices. Improved surgical techniques should enable a reduction in the complication rate.
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