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Ulla Aapola, Katja Viitala, Hannamaria Potila, Eira-Maija Savonen, Anne Huhtala, Tytti Sarjala, Hannu Uusitalo; Endophytic fungus Phialophora lignicola Extract Efficiently Protects ARPE-19 Cells against Oxidative Stress . Invest. Ophthalmol. Vis. Sci. 2011;52(14):5656.
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© ARVO (1962-2015); The Authors (2016-present)
Endophytic fungi are micro-organisms living asymptomatically inside the tissues of a host plant and are supposed to help the host surviving in hard environmental conditions thus making them an interesting source for new bioactive therapeutic compounds. The present study was undertaken to search for novel fungal bioactive substances and also to test their potential to oxidative damage in ARPE-19 cells as an in vitro model of age-related macular regeneration.
26 DNA-sequenced fungal species from the roots of Scots pine seedlings growing on Finnish peatlands were cultivated as pure cultures on MMN2 or Hagem plates. Collected grinded fungal material was extracted using hot water, PBS or 70 % ethanol. Total phenolic compounds (Folin-Ciocalteu’s phenol test), and ferric reduction/antioxidant power (FRAP-test) of the extracts were tested to screen antioxidant potential. Extracts were further tested in oxidative stress cell model by exposing the ARPE-19 cells to hydrogen peroxide and evaluating cytotoxicity with WST-1 assay (Roche). A known plant-derived antioxidant, quercetin, was used as a positive control.
The phenolic contents (≤ 46±2 GAE mg/g) and the FRAP-values (≤ 228±10 FeSO4 µmol/g) of the fungal extracts showed relatively high antioxidant potential. Out of 26 tested fungal species extract from Phialophora lignicola efficiently protected ARPE-19 cells against oxidative damage in cytotoxicity assay. The level of protection was dose dependent and comparable to the efficacy of quercetin.
An endophytic fungus Phialophora lignicola isolated from roots of Scots pine living in hard environmental conditions contains powerful antioxidant(s) protecting RPE cells against oxidative damage. The further characterization of the compound(s) and demonstration of the therapeutic potential in other disease models are on-going.
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