April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Corneal Intrastromal Prophylactic Antibiotics as an Adjunct in Cataract and Refractive Surgery Using High Performance Liquid Chromatography
Author Affiliations & Notes
  • Joseph J. Ma
    Ophthalmology, University of Toronto, Toronto, Ontario, Canada
  • Jieyun J. Huang
    Ophthalmology, University of Toronto, Toronto, Ontario, Canada
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5677. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Joseph J. Ma, Jieyun J. Huang; Corneal Intrastromal Prophylactic Antibiotics as an Adjunct in Cataract and Refractive Surgery Using High Performance Liquid Chromatography. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5677.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To quantify antibiotic concentrations acheivable in the anterior chamber of the eye using various methods of intrastromal corneal antibiotic administration.

Methods: : An in vitro model for the detection and quantification of topical, intracameral and intrastromal antibiotic administration (penetrating incision and non-penetrating pocket) using human corneal-scleral rims and high performance liquid chromotography was developed and validated. Time-concentration curves were obtained for various methods of corneal intrastromal antibiotic administration as well as standard methods for the administration of antibiotic.

Results: : Intrastromal antibiotic hydration of a cataract surgery incision resulted in a peak anterior chamber antibiotic concentration immediately after administration and remained above the MIC90 for most reported clinically significant endo-ophthalmitis isolates (302 ng/mL) until approximately 10 hrs after administration. Intrastromal hydration of a pocket (non-penetrating) corneal incision resulted in a peak anterior chamber concentration at approximately 3 hrs after administration (21600 ng/ml) and remained above the MIC90 of most reported clinically significant endophthalmitis isolates until 11 hrs (213 ng/ml). Concentration-time curves were compared to intracameral and topical antibiotic administration.

Conclusions: : Intrastromal corneal administration of antibiotics may be a useful adjunct method for antibiotic prophylaxis in the setting of cataract and refractive surgery.

Keywords: cataract • antibiotics/antifungals/antiparasitics • bacterial disease 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×