Purchase this article with an account.
Joseph J. Ma, Jieyun J. Huang; Corneal Intrastromal Prophylactic Antibiotics as an Adjunct in Cataract and Refractive Surgery Using High Performance Liquid Chromatography. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5677.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To quantify antibiotic concentrations acheivable in the anterior chamber of the eye using various methods of intrastromal corneal antibiotic administration.
An in vitro model for the detection and quantification of topical, intracameral and intrastromal antibiotic administration (penetrating incision and non-penetrating pocket) using human corneal-scleral rims and high performance liquid chromotography was developed and validated. Time-concentration curves were obtained for various methods of corneal intrastromal antibiotic administration as well as standard methods for the administration of antibiotic.
Intrastromal antibiotic hydration of a cataract surgery incision resulted in a peak anterior chamber antibiotic concentration immediately after administration and remained above the MIC90 for most reported clinically significant endo-ophthalmitis isolates (302 ng/mL) until approximately 10 hrs after administration. Intrastromal hydration of a pocket (non-penetrating) corneal incision resulted in a peak anterior chamber concentration at approximately 3 hrs after administration (21600 ng/ml) and remained above the MIC90 of most reported clinically significant endophthalmitis isolates until 11 hrs (213 ng/ml). Concentration-time curves were compared to intracameral and topical antibiotic administration.
Intrastromal corneal administration of antibiotics may be a useful adjunct method for antibiotic prophylaxis in the setting of cataract and refractive surgery.
This PDF is available to Subscribers Only