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Ichiro Tanano, Taiji Nagaoka, Tsuneaki Omae, Shinichi Otani, Akitoshi Yoshida; Unoprostone Isopropyl Elicits Dilation Of Isolated Porcine Arterioles. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6049.
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© ARVO (1962-2015); The Authors (2016-present)
Unoprostone isopropyl is a docosanoid that has been introduced for the treatment of glaucoma, under the name Rescula. Though, unoprostone isopropyl has been reported to increase ocular blood flow, it is still unclear whether unoprostone isopropyl is associated with retinal microcirculation. Herein, we examined the direct effect and the underlying mechanism of the vasomotor action of unoprostone isopropyl in retinal arterioles.
Porcine retinal arterioles (60-90 µm in internal diameter) were isolated, cannulated and pressurized (55 cmH2O) without flow for in vitro study. Videomicroscopic techniques were employed to record diameter change in response to unoprostone isopropyl.
Retinal arterioles dilated dose-dependently in response to unoprostone isopropyl (10-10-10-4 M). The nitric oxide (NO) synthase inhibitor N-nitro-L-arginine methyl ester, L-NAME (10 µM) markedly inhibited the vasodilation (p<0.0001). A nonselective K channels blocker, tetraethylammonium (TEA), inhibited the vasodilation (p<0.0001).
Unoprostone isopropyl elicits dilation of retinal arterioles in dose-dependent manner. The present findings suggest that vasodilatory effects induced by unoprostone isopropyl are mediated by the released NO and the activation of K channels, respectively.
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