April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Carbonic Anhydrase Inhibitors Induce Vasorelaxation in Isolated Intraocular Porcine Ciliary Arteries
Author Affiliations & Notes
  • Sidse Kringelholt
    Department of Ophthalmology, Aarhus University Hospital, Aarhus C, Denmark
  • Ulf Simonsen
    Department of Pharmacology, Aarhus University, Aarhus C, Denmark
  • Toke Bek
    Department of Ophthalmology, Aarhus University Hospital, Aarhus C, Denmark
  • Footnotes
    Commercial Relationships  Sidse Kringelholt, None; Ulf Simonsen, None; Toke Bek, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6052. doi:
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      Sidse Kringelholt, Ulf Simonsen, Toke Bek; Carbonic Anhydrase Inhibitors Induce Vasorelaxation in Isolated Intraocular Porcine Ciliary Arteries. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6052.

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Abstract

Purpose: : Carbonic anhydrase inhibitors (CAIs) are widely used to reduce the intraocular pressure in glaucoma, but CAIs have also been shown to have vasoactive effects on the retinal and the extraocular long posterior ciliary vessels. However, the effect of CAIs on the smaller intraocular ciliary artery branches supplying the ciliary body has not been studied in detail.

Methods: : A new technique for isolating and mounting the intraocular part of porcine ciliary arteries in a myograph system for isometric tension recording was developed. The vascular tone was recorded during concentration-response experiments with the CAIs acetazolamide and dorzolamide. The CAIs were added in nine concentration steps in the interval between 10-6 and 10-2 M after precontraction with the tromboxane analogue U46619 10-7 M.

Results: : Preliminary experiments showed that both examined CAIs induced a significant vasorelaxation of ciliary arteries. This effect was significant (p<0.05) for the two highest concentrations of acetazolamide and for the seven highest concentrations of dorzolamide, suggesting a different potency of these two CAIs.

Conclusions: : Carbonic anhydrase inhibitors have a vasorelaxing effect on intraocular porcine ciliary arteries in vitro. Therefore, the mechanism of action of CAIs in the treatment of glaucoma may involve several mechanims, both including changes in aqueous humor production and changes in the blood flow to the ciliary body.

Keywords: ciliary body • blood supply • carbonic anhydrase 
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