Purchase this article with an account.
Heinrich Gerding, Melanie Timmermann; Staining Of The Internal Limiting Membrane With A Novel Heavy Brilliant Blue G-solution. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6109.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the hydrodynamic properties, clinical efficacy and safety of a novel heavier-than-water dye (BBG-D2O) designed for staining of the internal limiting membrane (ILM) during vitreoretinal procedures, consisting of brilliant blue G (BBG, 0.25 mg/ml) with the addition of 13 % of deuterium oxide (D2O).
The gravity related hydrodynamic distribution of BBG-D2O was analysed in 7 experiments using standard test cuvettes filled with balanced salt solution (BSS) and compared to conventional BBG. Clinically BBG-D2O was used in 15 patients (mean age: 74.4 +/- 5.1 years) with epiretinal membranes (ERM)) for staining of the ILM during surgery. Removed ERM and ILM were processed for light microscopic analysis.
Compared to standard solutions of BBG the heavy preparation of the dye (BBG-D2O) presented a rapid flow towards the bottom of test cuvettes. After 15 seconds the concentration of BBG in the lowest 25 % of the cuvettes was 4.2 +/- 0.2 µg/ml (mean +/- standard deviation) compared to 19.0 +/- 2.6 µg/ml of BBG-D2O (p<0.005). Clinically BBG-D2O resulted in a strong staining effect of the ILM, comparable to conventional indocyanine green (ICG) solution and clearly more intensive than that of conventional BBG solution of the same concentration (0.25 mg/ml). The intensive staining effect of primary applied BBG-D2O (before ERM removal) allowed in the majority of cases (10 of 15) an en bloc removal of ILM and ERM together as a one-step procedure. Removal of the ILM was histopathologically confirmed in all cases. No signs of adverse effects related to the use of BBG-D2O were observed during a follow-up period of at least 2 months.
Heavy brilliant blue G (BBG-D2O ) provides a significantly improved staining effect of the ILM and by this makes ILM peeling more efficient and easier.
This PDF is available to Subscribers Only