April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Ultrastructural Features Of Epiretinal Membrane Associated With Long-standing Retained Perfluorocarbon Liquid
Author Affiliations & Notes
  • SongEun Lee
    Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York
  • Louis K. Chang
    Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York
  • Peter Gouras
    Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York
  • Stanley Chang
    Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York
  • Footnotes
    Commercial Relationships  SongEun Lee, None; Louis K. Chang, None; Peter Gouras, None; Stanley Chang, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6145. doi:
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    • Get Citation

      SongEun Lee, Louis K. Chang, Peter Gouras, Stanley Chang; Ultrastructural Features Of Epiretinal Membrane Associated With Long-standing Retained Perfluorocarbon Liquid. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6145.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To describe ultrastructural features of an epiretinal membrane(ERM) from an eye with retained perfluorocarbon liquid (PFCL)from a retinal detachment repair 15 years ago.

 
Methods:
 

A 59-year-old woman underwent 23-gauge pars plana vitrectomywith membrane peeling to remove a thick, irregular ERM. Thespecimen was fixed in 2% glutaraldehyde, and reviewed underthe transmission electron microscope (EM) after routine tissueprocessing.

 
Results:
 

The EM showed multilayered membranes with internal limitingmembrane and one to three thick collagen layers (up to 10µm)with interposition of cell layer and dead cell debris. The celllayers were mainly composed of fibrous glial cells and fibroblastlike cells containing numerous vacuoles in the cytoplasm.

 
Conclusions:
 

The EM of an ERM from an eye with long-standing retained PFCLrevealed unique ultrastructural features. The thick multilayeredcollagen and the vacuole containing cells are very unusual findingsin ERM. The contents inside vacuoles in the cells are likelyPFCL. We hypothesize that PFCL may cause long-standing, subclinicalinflammation stimulating fibroblast like cells to produce collagen,contributing to the development of the ERM.  

 

 
Keywords: vitreous substitutes • pathology: human • extracellular matrix 
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