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Jody A. Summers Rada, Nicole A. Datson, David Troilo; Early Changes in Gene Expression in RPE/Choroid of Marmoset Eyes in Response to Image Defocus. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6300.
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Several studies suggest that a cascade of chemical events, originating from the retina and terminating at the sclera is responsible for the regulation of postnatal ocular growth and refraction in response to visual input. A microarray analysis was employed to identify genes that were up- and down- regulated in the RPE/choroids of marmoset eyes during early exposure to induced myopic and hyperopic defocus.
Total RNA was isolated from the choroid/RPEs of treated and control eyes of 10 common marmosets (Callithrix jacchus) following +5D/plano (n = 5) or -5D/plano (n = 5) contact lens treatment for 10 days. After reverse transcription, cDNA was labeled and hybridized to marmoset-specific oligonucleotide microarrays (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts (a total of 20 arrays).
10 days of -5 or +5 contact lens treatment resulted in negligible changes in vitreous chamber depth (VC) as compared to controls (dVC: -.0276 ± 0.0363 mm; -.0064 ± .0234 mm, respectively). Extremely small differences in gene expression were detected in RPE/choroids from treated eyes as compared with controls (ranging from -1.32 - +1.39 fold differences). Forty genes had raw p-values ≤ 0.05 for the minus lens comparison of treated versus control eyes and 36 genes had raw p-values ≤ 0.05 for the plus lens comparison of treated versus control eyes. No genes were found to be significantly differentially expressed for either of the two comparisons after correction for multiple testing. Of the genes with raw p-values ≤0.05, only one gene, toll- like receptor 6 (Tlr6), was bidirectionally regulated (+1.15 fold in minus lens-treated eyes and -1.12 fold in plus lens-treated eyes (p≤0.002).
Relatively small differences in gene expression are induced in the choroid/RPE in response to myopic or hyperopic defocus prior to measureable changes in ocular size. The identified genes may lead to novel insights into choroid/RPE function under normal and diseased conditions.
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