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Tomohiko Usui, Tetsuya Toyono, Seiichi Yokoo, Shiro Amano; Role of Angptl2 in Corneal Angiogenesis and Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6387.
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© ARVO (1962-2015); The Authors (2016-present)
Angiopoietin-like proteins (Angptls) are secreted proteins possessing an amino-terminal coiled-coil domain and a carboxyl-terminal fibrinogen-like domain, which are similar to angiopoietins. Among the Angptl family, Angptl2 may be involved in angiogenesis and inflammation. We have previously identified that expression of Angptl2 was increased in mice inflammatory corneal neovascularization models. The purpose of this study was to determine the role of Angptl2 in inflammatory corneal angiogenesis and lymphangiogenesis.
Corneal angiogenesis and lymphangiogenesis was induced by suturing 10-0 nylon 1mm away from limbal vessels in Angptl2 knockout(Angptl2-/-) mice and wild-type. The areas of angiogenesis and lymphangiogenesis on flatmout of corneas were observed by CD31 (vascular endhothelial marker) and LYVE-1 (lymphatic endothelial marker) immunohistochemical staining 7days after suturing.
Corneal angiogenesis area in Angptl2-/- mice was significantly less than that in wild type mice (9.47±3.95% vs 17.45±2.70%, p=0.01). Similary, corneal lymphamgiogenesis area in Angptl2-/- mice developed less than that in wild type mice (1.85±0.66% vs 3.20±0.63%, p=0.02)
Angptl2 might be proangiogenic as well as prolymphangiogenic factor in inflammatory corneal diseases
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