April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Inflammatory Cytokine Profile In Aqueous Of Tuberculous Uveitis
Author Affiliations & Notes
  • Jing Li
    Singapore Eye Research Inst, Singapore, Singapore
    Dept. of ophthalmology, Uveitis,
    National University of Singapore, Singapore, Singapore
  • Marcus Ang
    Singapore National Eye Centre, Singapore, Singapore
  • Chui Ming Gemmy Cheung
    Singapore National Eye Centre, Singapore, Singapore
  • Maya Vania
    Singapore Eye Research Inst, Singapore, Singapore
  • Soon-Phaik Chee
    Dept. of ophthalmology, Uveitis,
    Dept. of Ophthalmology,
    National University of Singapore, Singapore, Singapore
    Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships  Jing Li, None; Marcus Ang, None; Chui Ming Gemmy Cheung, None; Maya Vania, None; Soon-Phaik Chee, None
  • Footnotes
    Support  This study is supported by National Medical Research Council (NMRC) of Singapore: NMRC/EDG/0050/2009 to Dr. J. Li, and Biomedical Research Council of Singapore (BMRC) grant: SIgN 09-026 to Dr. S.P. Ch
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6633. doi:
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      Jing Li, Marcus Ang, Chui Ming Gemmy Cheung, Maya Vania, Soon-Phaik Chee; Inflammatory Cytokine Profile In Aqueous Of Tuberculous Uveitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6633.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the cytokine profile in the aqueous humour of patients with tuberculosis (TB)-associated uveitis (TAU).

Methods: : Aqueous humour samples were collected from 12 eyes of 12 patients with TAU, 8 idiopathic acute anterior uveitis (AAU) patients in the uveitis clinic and 23 cataract subjects during phacoemulsification surgery in this prospective case-controlled study. TAU was diagnosed using recognized criteria: positive Mantoux test, positive response to anti-TB treatment and absence of underlying diseases that could cause uveitis. A multiplexed cytokine analysis was used to quantify the presence of 20 proinflammatory cytokines in the aqueous.

Results: : Mean age of TAU patients was 51 years (range 16-69 yrs), mean age of AAU patients was 54 years (range 33-79 yrs) and mean age of cataract patients was 66 years (range 25-89 yrs). Principal component analysis of cytokine profile revealed separated clusters for TAU and cataract aqueous samples. Significant differences were found in the concentration of CCL3 (MIP-1α) (3.02±1.01 vs 0.76±0.26, mean±std err, p=0.007), CCL5 (RANTES) (39.13±12.83 vs 7.54±1.36, p=0.004) and CXCL10 (IP-10) (57739.8±19982.3 vs 360.5±217.1, p=0.001) between TAU and cataract patients samples. Between TAU aqueous samples and idiopathic AAU sample, significant differences were found in the concentrations of interleukin-2 (IL-2) (3.6±0.52 vs 0.0±0.00, p=0.03), IL-7 (1.6±0.23 vs 9.0±2.93, p=0.001), IL-12 (7.4±1.64 vs 0.3±0.26, p=0.018), tumor necrosis factor-α (TNF-a) (15.5±2.07 vs 3.7±3.07, p=0.006) and interferon-α2 (IFNα2) (35.8±7.46 vs 2.0±1.28, p=0.025).

Conclusions: : TAU patients exhibit different aqueous cytokine profiles than idiopathic AAU or cataract patients.

Keywords: uveitis-clinical/animal model • inflammation • cytokines/chemokines 
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