April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
C1q Deficiency Decreases Glaucomatous RGC Loss
Author Affiliations & Notes
  • Eman Elhawy
    Ophthalmology,
    Suny Downstate Medical Center, Brooklyn, New York
  • Lizhen Ren
    Cell Biology,
    Suny Downstate Medical Center, Brooklyn, New York
  • Shane Scanlon
    Cell Biology,
    Suny Downstate Medical Center, Brooklyn, New York
  • Konstantin Astafurov
    Cell Biology,
    Suny Downstate Medical Center, Brooklyn, New York
  • John Danias
    Ophthalmology, Cell Biology,
    Suny Downstate Medical Center, Brooklyn, New York
  • Footnotes
    Commercial Relationships  Eman Elhawy, None; Lizhen Ren, None; Shane Scanlon, None; Konstantin Astafurov, None; John Danias, None
  • Footnotes
    Support  NEI R01 EY15224
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6675. doi:
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    • Get Citation

      Eman Elhawy, Lizhen Ren, Shane Scanlon, Konstantin Astafurov, John Danias; C1q Deficiency Decreases Glaucomatous RGC Loss. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6675.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether absence of C1q affects glaucomatous RGC loss

Methods: : C1q knockout (KO) congenic DBA/2 mice were generated by breeding into the D2 strain for 10 generations and were then allowed to age. IOP of a subgroup of male KOs and heterozygote littermates were recorded from the 6th to the 12th month of age. RGCs of 7 KOs and 5 heterozygotes males were retrogradely labeled at 12 months and counted after imaging flat mounts. Total retinal area was measured.

Results: : Mean, peak and cumulative IOP exposure were similar in heterozygote and KO eyes (p>0.78 t-test). Mean RGC counts were ~29.6K for heterozygotes and ~40.3K for KOs (p>0.28 t-test, power 0.18). RGC loss in the least affected eye of each animal was more severe (RGC counts of less than 35K) in heterozygotes than KOs (p<0.03, Fisher’s exact). Total retinal area was significantly larger in heterozygotes compared to KOs (p<0.04, t-test). RGC counts correlated weakly (inversely) with mean IOP (R2=0.41) while total retinal area correlated very weakly (also inversely) with maximum IOP (R2=0.24).

Conclusions: : C1q KOs in the DBA/2 background have slightly decreased glaucomatous RGC loss and decreased retinal area compared to heterozygote littermates. IOP differences could not account for these differences.

Keywords: ganglion cells • inflammation • retina: proximal (bipolar, amacrine, and ganglion cells) 
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