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Eman Elhawy, Lizhen Ren, Shane Scanlon, Konstantin Astafurov, John Danias; C1q Deficiency Decreases Glaucomatous RGC Loss. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6675.
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© ARVO (1962-2015); The Authors (2016-present)
To determine whether absence of C1q affects glaucomatous RGC loss
C1q knockout (KO) congenic DBA/2 mice were generated by breeding into the D2 strain for 10 generations and were then allowed to age. IOP of a subgroup of male KOs and heterozygote littermates were recorded from the 6th to the 12th month of age. RGCs of 7 KOs and 5 heterozygotes males were retrogradely labeled at 12 months and counted after imaging flat mounts. Total retinal area was measured.
Mean, peak and cumulative IOP exposure were similar in heterozygote and KO eyes (p>0.78 t-test). Mean RGC counts were ~29.6K for heterozygotes and ~40.3K for KOs (p>0.28 t-test, power 0.18). RGC loss in the least affected eye of each animal was more severe (RGC counts of less than 35K) in heterozygotes than KOs (p<0.03, Fisher’s exact). Total retinal area was significantly larger in heterozygotes compared to KOs (p<0.04, t-test). RGC counts correlated weakly (inversely) with mean IOP (R2=0.41) while total retinal area correlated very weakly (also inversely) with maximum IOP (R2=0.24).
C1q KOs in the DBA/2 background have slightly decreased glaucomatous RGC loss and decreased retinal area compared to heterozygote littermates. IOP differences could not account for these differences.
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