April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Taurine Induced Effects On The Tear Proteome Of Dry Eye Patients And Contact Lens Wearers Using A RP-RP-2D-Capillary-LC-MALDI-TOF/TOF MS Approach
Author Affiliations & Notes
  • Sebastian Funke
    Experimental Ophthalmology, University Medical Center, Mainz, Germany
  • Diana Azimi
    Experimental Ophthalmology, University Medical Center, Mainz, Germany
  • Norbert Pfeiffer
    Experimental Ophthalmology, University Medical Center, Mainz, Germany
  • Franz H. Grus
    Experimental Ophthalmology, University Medical Center, Mainz, Germany
  • Footnotes
    Commercial Relationships  Sebastian Funke, None; Diana Azimi, None; Norbert Pfeiffer, None; Franz H. Grus, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6680. doi:
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      Sebastian Funke, Diana Azimi, Norbert Pfeiffer, Franz H. Grus; Taurine Induced Effects On The Tear Proteome Of Dry Eye Patients And Contact Lens Wearers Using A RP-RP-2D-Capillary-LC-MALDI-TOF/TOF MS Approach. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6680.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Tear proteomic studies revealed distinct similarities between contact lens wearers and dry eye patients coinciding with the finding that contact lens wearers often develop dry eye symptoms. AMO Complete multipurpose contact lens cleaning solutions containing Taurine seem to have a beneficial effect on the pathology of contact lens induced dry eye. Therefore we focus on Taurine as a predominant ingredient of those contact lens cleaning solutions and developed a mass spectrometric based strategy to illuminate the effects of Taurine on the tear proteome of contact lens wearers and sicca patients.

Methods: : Three experimental groups were monitored (N=4 individuals/group). Two contact lens wearer groups were examined; one group was using eye drops containing 0.05% Taurine, the other was using for control physiological Sodium Chloride solution. Also, a third group of sicca patients used Taurine solution. Individual tears were collected at six time points over five weeks. Tear pools of each group per time point were analyzed using 1D-SDS PAGE combined with a Reversed Phase-Reversed Phase-2D-capillary-LC MALDI TOF/TOF MS approach. Identified proteins were quantified by an in-house developed label-free quantification approach.

Results: : In summary 267 different tear proteins were identified. We found a subset of more than 20 proteins showing a strong linear Taurine response with r2 values>0.5. Most Taurine response effects were found in the contact lens group demonstrated by significant linear up trends (p<0.05). "Prolactin-inducible protein" (Mowse Score 2998) as a dry eye associated protein involved in multiple immune processes and "Transcription Elongation factor B3" (Mowse Score 129) are distinctly responding to the use of Taurine in the contact lens group (r2 > 0.7). "Deleted in malignant brain tumors 1 protein" (Mowse Score 1305) shows Taurine response in the sicca group.

Conclusions: : We were able to get a deep insight of the tear proteome and found proteins clearly responding to the application of Taurine. A strong Taurine influence was found in the contact lens wearer group. We think that these proteins can be focused on in further studies and could have a beneficial effect in contact lens cleaning solutions.

Keywords: proteomics • contact lens • taurine 
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