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Fan Yuan, Lucinda J. Camras, Pedro Gonzalez; Trabecular Meshwork Stiffness in Ex Vivo Perfused Porcine Eyes. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6693.
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The goal of the study was to determine if the outflow facilitywas dependent on trabecular meshwork (TM) stiffness in perfusedporcine eyes, and whether the dependence varied with post-mortemtime.
Porcine eyes were obtained within 1-2 hrs post-mortem and maintainedat 4°C until use. Whole eyes were perfused at a constantpressure of 15 mmHg for 30 min to determine outflow facility(C). The TM was then removed for mechanical analysis using atomicforce microscopy (AFM) within 3-6 hrs after perfusion. A probewith gold-coated colloid of 5 µm in diameter was usedto indent the tissue at a velocity of 5 µm/s. The Hertzequation was used to calculate the elastic modulus (E). Thevalues of C and E were measured over a range of 3-32 and 6-38hrs post-mortem, respectively. TM tissues were also assessedfor any morphological changes.
The average values of C and E were 0.23 ± 0.02 µL/min/mmHgand 1.38± 0.47 kPa (n=8), respectively. There were nocorrelations between: (a) E vs. C (R2=0.127), (b) the post-mortemtime vs. C (R2=0.201), and (c) time post-mortem time vs. E (R2=0.440).No significant morphological changes were observed.
TM stiffness did not correlate to outflow facility in perfusedporcine eyes ex vivo. Likewise, the post-mortem time did nothave a significant effect on outflow facility and TM stiffnesswithin the experimental periods reported above.
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