April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Trabecular Meshwork Stiffness in Ex Vivo Perfused Porcine Eyes
Author Affiliations & Notes
  • Fan Yuan
    Biomedical Engineering,
    Duke University, Durham, North Carolina
  • Lucinda J. Camras
    Biomedical Engineering,
    Duke University, Durham, North Carolina
  • Pedro Gonzalez
    Ophthalmology, Duke Eye Center,
    Duke University, Durham, North Carolina
  • Footnotes
    Commercial Relationships  Fan Yuan, None; Lucinda J. Camras, None; Pedro Gonzalez, None
  • Footnotes
    Support  NEI EY016228, NEI EY01894, NEI EY05722, RPB
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6693. doi:
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      Fan Yuan, Lucinda J. Camras, Pedro Gonzalez; Trabecular Meshwork Stiffness in Ex Vivo Perfused Porcine Eyes. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6693.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

The goal of the study was to determine if the outflow facilitywas dependent on trabecular meshwork (TM) stiffness in perfusedporcine eyes, and whether the dependence varied with post-mortemtime.

 
Methods:
 

Porcine eyes were obtained within 1-2 hrs post-mortem and maintainedat 4°C until use. Whole eyes were perfused at a constantpressure of 15 mmHg for 30 min to determine outflow facility(C). The TM was then removed for mechanical analysis using atomicforce microscopy (AFM) within 3-6 hrs after perfusion. A probewith gold-coated colloid of 5 µm in diameter was usedto indent the tissue at a velocity of 5 µm/s. The Hertzequation was used to calculate the elastic modulus (E). Thevalues of C and E were measured over a range of 3-32 and 6-38hrs post-mortem, respectively. TM tissues were also assessedfor any morphological changes.

 
Results:
 

The average values of C and E were 0.23 ± 0.02 µL/min/mmHgand 1.38± 0.47 kPa (n=8), respectively. There were nocorrelations between: (a) E vs. C (R2=0.127), (b) the post-mortemtime vs. C (R2=0.201), and (c) time post-mortem time vs. E (R2=0.440).No significant morphological changes were observed.

 
Conclusions:
 

TM stiffness did not correlate to outflow facility in perfusedporcine eyes ex vivo. Likewise, the post-mortem time did nothave a significant effect on outflow facility and TM stiffnesswithin the experimental periods reported above.  

 

 
Keywords: trabecular meshwork • outflow: trabecular meshwork • anterior segment 
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