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K. Takeuchi, M. Nakazawa, Y. Ebina, Y. Miyagawa, H. Yamazaki, T. Metoki, T. Ito, Y. Yokoi; Inhibitory Effects of Trehalose on Fibroblasts and Implications for Ocular Surgery. Invest. Ophthalmol. Vis. Sci. 2009;50(13):464.
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To investigate inhibition of trehalose on proliferation of fibroblasts.
Normal human dermal fibroblasts (NHDF) and Normal human epidermal keratinocytes (NHEK) were cultured with trehalose-containing medium (40/20/10/5/2.5/ 1.25/0.06%). The growth activity of the cells was evaluated by MTT assay and Diff Quik staining. In addition, after simple conjunctival incision on rabbits, trehalose-containing eye drops were instilled on the operated eyes and the condition of fibroblasts and conjunctiva were evaluated by immunohistochemistry and Masson trichrome staining.Futhermore, we performed trabeculectomy on rabbits. We divided them into 4 groups (5% or 10% trehalose eye drop group after operation/ 0.9% saline eye drop group after operation/ 0.04% MMC using group while operation). After the operation, we measured intraocular pressure (IOP) continuously, and we enucleated eye balls, and observed the extent of adhesion by Mason trichrome staining.
Growth activities of fibroblasts and keratinocytes were inhibited by dose-dependent manner. Fibroblasts were strongly inhibited in the concentration of more than 5% trehalose, but keratinocytes were less inhibited than fibroblasts.In vivo, the trehalose group demonatrated less firm adhesion between conjunctiva and sclera compared to the control. Also, after trabeculectomy, low IOP was maintained at lower pressure than the control for a longer period.
Trehalose is a kind of saccharides, and isomer with sucrose. The osmotic pressure of 5% trehalose medium equal to 0.9% saline solution. We think that more than 10% trehalose-containing sodium is concerned with the osmotic pressure. But, from the above results, we think that trehalose has the growth inhibitory effect on fibroblasts by not only osmotic pressure but other mechanisms.Futhermore, because trehalose is antigenically inert, we concluded that trehalose has the possibility of the new medicine which can be used after glaucoma surgery in the future.
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