April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Comparative Evaluation of Corneal Residual Beds Created by Two Generation Femtosecond Laser Using Scanning Electron Microscopy
Author Affiliations & Notes
  • M. A. Guenena
    Ophthalmology, Magill Research Center, Storm Eye Instit, Charleston, South Carolina
  • H. P. Sandoval
    Ophthalmology, Magill Research Center, Storm Eye Instit, Charleston, South Carolina
  • K. D. Solomon
    Ophthalmology, Magill Research Center, Storm Eye Instit, Charleston, South Carolina
  • Footnotes
    Commercial Relationships  M.A. Guenena, None; H.P. Sandoval, None; K.D. Solomon, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 550. doi:https://doi.org/
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      M. A. Guenena, H. P. Sandoval, K. D. Solomon; Comparative Evaluation of Corneal Residual Beds Created by Two Generation Femtosecond Laser Using Scanning Electron Microscopy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):550. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the smoothness of the corneal residual bed after flap creation by a 5th generation femtosecond laser (iFS Advanced Femtosecond Laser) and a previous generation (IntraLase®) using scanning electron microscopy (SEM).

Methods: : A corneal flap was created in 8 fresh cadaver eyes using a 5th generation femtosecond laser and a previous version. Round and elliptical shape flaps were created with 100 &110 µm depth. Residual beds were fixed using 2% cacodylate glutaraldehyde and processed for SEM evaluation. The residual bed surfaces as well as the edges were examined. Standardized photographs were taken and compared.

Results: : Mean donor age was 78.0 ±9.0 years. Mean time from dead to preservation was 9:15 ±5:17 hours. Stromal beds in the 5th generation group were smoother, edges were more oblique and fewer irregularities were seen at the interface compared to the previous model.

Conclusions: : The higher repetition rate (150 KHz) used in the 5th generation femtosecond laser allows for tighter spot separation. This leads to the use of less energy and lowers tissue response. Thus helps creating smoother stromal bed that may lead to better surgical outcome.

Keywords: microscopy: electron microscopy • refractive surgery: comparative studies 
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