April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Goblet Cell Response to Photorefractive Keratectomy: Effect on Total and Filled Goblet Cell Number
M. Shatos; D. A. Sediq; K. S. Bower; J. D. Edwards; L. Peppers; C. D. Coe; D. Dartt
Author Affiliations & Notes
  • M. Shatos
    Ophthal/Harvard Med Sch, Schepens Eye Research Institute, Boston, Massachusetts
  • D. A. Sediq
    Ophthalmology, Walter Reed Army Medical Center, Washington, Dist. of Columbia
  • K. S. Bower
    Ophthalmology, Walter Reed Army Medical Center, Washington, Dist. of Columbia
  • J. D. Edwards
    Ophthalmology, Walter Reed Army Medical Center, Washington, Dist. of Columbia
  • L. Peppers
    Ophthalmology, Walter Reed Army Medical Center, Washington, Dist. of Columbia
  • C. D. Coe
    Ophthalmology, Walter Reed Army Medical Center, Washington, Dist. of Columbia
  • D. Dartt
    Ophthal/Harvard Med Sch, Schepens Eye Research Institute, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  M. Shatos, None; D.A. Sediq, None; K.S. Bower, None; J.D. Edwards, None; L. Peppers, None; C.D. Coe, None; D. Dartt, None.
  • Footnotes
    Support  Department of Defense Grant W81XWH-04-2-0008
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 574. doi:
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      M. Shatos, D. A. Sediq, K. S. Bower, J. D. Edwards, L. Peppers, C. D. Coe, D. Dartt; Goblet Cell Response to Photorefractive Keratectomy: Effect on Total and Filled Goblet Cell Number. Invest. Ophthalmol. Vis. Sci. 2009;50(13):574.

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Abstract

Purpose: : To determine if filled GC represent the total conjunctival GC population and if PRK alters the number of these cells.

Methods: : Impression cytology samples (ICS) were taken from the superior and temporal conjunctivae of 8 patients (3 males, 5 females; average age 30 yr) before and 1M after PRK. Tear film status was evaluated by Schirmer test (ST) without anesthesia, tear breakup time (TBUT), and McMonnies Questionnaire (MQ). ICS were transferred to glass slides and stained with anti-keratin 7 (K7), marks GC bodies; Helix pomatia agglutinin (HPA), marks GC secretory product; and DAPI, marks cell nuclei. Five areas were counted for each sample. The total number of cells was determined by counting DAPI stained nuclei, total number of GC(filled and empty) by determining the number of DAPI stained nuclei positive for K7, and number of filled GC by measuring the number of DAPI stained nuclei positive for both K7 and HPA.

Results: : Mean spherical equivalent pre-op was -2.99+/-0.35 diopters. Average ablation depth for the 8 patients was 48.4+/-5.2µ. ST value was 21.3+/-2.7 mm before surgery and decreased to 16.4+/-2.5 mm 1M post-op. Average TBUT significantly decreased from 20.1+/-2.4 sec pre-op to 11.5+/-1.5 sec at 1M post-op. Score on MQ increased from 5.9+/-1.1 pre-op to 8.0+/-1.7 post-op. Total number of GC did not differ from the number of filled GC in the superior conjunctiva, being 146+/-29 and 121+/-25 respectively pre-op and 61+/-15 and 52+/-14 post-op. Similar results were obtained in the temporal ICS. Both total number and number of filled GC in the superior, but not temporal, conjunctiva significantly decreased post-op compared to pre-op. Total number of cells decreased from 146+/-29 to 61+/-15; number of filled GC decreased from 121+/-25 to 52+/-14.

Conclusions: : There is not a significant population of unfilled GC in the conjunctiva. PRK decreased the GC population in the superior, but not temporal, conjunctiva accompanied by changes consistent with surgically-induced dry eye.

Clinical Trial: : www.clinicaltrials.gov NCT00411827

Keywords: cornea: tears/tear film/dry eye • conjunctiva • refractive surgery: PRK 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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