April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Pathway Specific Gene Expression Profiling in a Laser-induced Choroidal Neovascularization (cnv) Mouse Model
Author Affiliations & Notes
  • P. Panda
    Laboratory of Immunology, NEI/NIH, Bethesda, Maryland
  • Z. Li
    Laboratory of Immunology, NEI/NIH, Bethesda, Maryland
  • R. B. Nussenblatt
    Laboratory of Immunology, NEI/NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  P. Panda, None; Z. Li, None; R.B. Nussenblatt, None.
  • Footnotes
    Support  NIH Clinical Research Training Program, a public-private partnership supported jointly by the NIH and Pfizer Inc
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1169. doi:
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    • Get Citation

      P. Panda, Z. Li, R. B. Nussenblatt; Pathway Specific Gene Expression Profiling in a Laser-induced Choroidal Neovascularization (cnv) Mouse Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1169.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : A strong correlation of inflammatory responses in age-related macular degeneration (AMD) and choroidal neovascularization (CNV) has been well documented. The laser-induced CNV mouse model has been widely used to study molecular mechanisms and potential therapeutic targets for the treatment of AMD and CNV. This study aims to profile early inflammatory gene expression in murine eyes with laser-induced CNV.

Results: : Housekeeping genes such as GAPDH and internal positive controls were most strongly expressed in the control group. Complement C2 (an integral part of the complement cascade), glomulin (Glmn, an FKBP associated protein involved in vascular development), and bone morphogenetic protein 6 (BMP6, involved in collagen metabolism) were found up-regulated in the 24 hour laser group.

Keywords: inflammation • immunomodulation/immunoregulation • choroid: neovascularization 
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