April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Monocyte Chemoattractant Protein (mcp-1/ccl2) Orchestrates Mast Cell-Mediated Allergic Inflammatory Responses
Author Affiliations & Notes
  • T. Tominaga
    Tottori University, Yonago, Japan
  • D. Miyazaki
    Tottori University, Yonago, Japan
  • K. Yakura
    Tottori University, Yonago, Japan
  • S. Namba
    Tottori University, Yonago, Japan
  • N. Komatsu
    Tottori University, Yonago, Japan
  • Y. Inoue
    Tottori University, Yonago, Japan
  • Footnotes
    Commercial Relationships  T. Tominaga, None; D. Miyazaki, None; K. Yakura, None; S. Namba, None; N. Komatsu, None; Y. Inoue, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1203. doi:
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      T. Tominaga, D. Miyazaki, K. Yakura, S. Namba, N. Komatsu, Y. Inoue; Monocyte Chemoattractant Protein (mcp-1/ccl2) Orchestrates Mast Cell-Mediated Allergic Inflammatory Responses. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1203.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Our whole genome analysis of transcriptome of acute allergic inflammation identified monocyte chemoattractant protein-1 (MCP-1/CCL2) as presumable disease- susceptible gene (IOVS 2008 in press). Using experimental murine allergic conjunctivitis model, we evaluated the roles of MCP-1 and its receptor, CCR2.

Methods: : The effect of CCR2 blockade was evaluated by in vitro isolated conjunctival mast cells. The role of MCP-1 in the conjunctiva was evaluated by subconjunctival injection of the recombinant MCP-1 protein into non-sensitized mice. Ragweed pollen-sensitized mice were provoked for acute allergic inflammation by the allergen instillation. Roles of MCP-1 were evaluated clinically and histologically by treatment of anti-MCP-1 antibody or specific CCR2 antagonist (RS 504393).

Results: : In vitro study revealed CCR2 blockade significantly suppressed mast cell degranulation (P<0.05). MCP-1 injection activated mast cell degranulation in non-sensitized mice. Inhibition of MCP-1/CCR2 significantly suppressed early phase clinical symptoms and allergen-specific mast cell degranulation (P<0.05) without affect on allergen-specific IgE, or Th2 cytokine release from isolated draining lymph node cells.

Conclusions: : MCP-1/CCR2 plays important roles in allergen-specific mast cell activation and acute phase inflammation in murine experimental allergic conjunctivitis.

Keywords: cytokines/chemokines • conjunctivitis • receptors 

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