April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
The Inhibitory Effect of IL-27 for Experimental Choroidal Neovascularization
Author Affiliations & Notes
  • E. Hasegawa
    Kyushu University, Fukuoka, Japan
  • Y. Oshima
    Kyushu University, Fukuoka, Japan
  • K.-H. Sonoda
    Kyushu University, Fukuoka, Japan
  • A. Takeda
    Kyushu University, Fukuoka, Japan
  • K. HIjioka
    Kyushu University, Fukuoka, Japan
  • T. Fujimoto
    Kyushu University, Fukuoka, Japan
  • T. Ishibashi
    Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships  E. Hasegawa, None; Y. Oshima, None; K.-H. Sonoda, None; A. Takeda, None; K. HIjioka, None; T. Fujimoto, None; T. Ishibashi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 834. doi:
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      E. Hasegawa, Y. Oshima, K.-H. Sonoda, A. Takeda, K. HIjioka, T. Fujimoto, T. Ishibashi; The Inhibitory Effect of IL-27 for Experimental Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2009;50(13):834.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : IL-27 is a member of the IL-6/IL-12 family cytokine playing an important role of Th1 differentiation and suppression of proinflammatory cytokine production. And IL-27 also exhibited the antiangiogenic activity on tumor-induced neovascularization. We investigated the role of IL-27 in the development of laser-induced choroidal neovascularization (CNV) using murine model.

Methods: : Adult wild-type C57BL/6J mice were used to investigate the effect of IL-27 on the laser-induced CNV model with or without intravitreous injection of IL-27 or anti IL-27 antibodies. RT-PCR from the eyes were performed 6, 12, 24, 48 hours after laser photocoagulation. The CNV was assessed on day 7 by histological examination and image analysis of flat mounts from mice perfused with fluorescein-lableled dextran.

Results: : Real-time PCR revealed that the RNA expression of IL-27 was significantly increased after 12hours and maintained for 24hours and 48hours after laser photocoagulation. The mice injected recombinant IL-27 had significantly smaller CNV than control mice. And this inhibitory effect on CNV by IL-27 was denied using anti IL-27 antibody intravitreous injection.

Conclusions: : These results suggested that IL-27 has the antiangiogenic activity in the development of laser-induced CNV.

Keywords: choroid: neovascularization • age-related macular degeneration 

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