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E. Hasegawa, Y. Oshima, K.-H. Sonoda, A. Takeda, K. HIjioka, T. Fujimoto, T. Ishibashi; The Inhibitory Effect of IL-27 for Experimental Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2009;50(13):834.
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IL-27 is a member of the IL-6/IL-12 family cytokine playing an important role of Th1 differentiation and suppression of proinflammatory cytokine production. And IL-27 also exhibited the antiangiogenic activity on tumor-induced neovascularization. We investigated the role of IL-27 in the development of laser-induced choroidal neovascularization (CNV) using murine model.
Adult wild-type C57BL/6J mice were used to investigate the effect of IL-27 on the laser-induced CNV model with or without intravitreous injection of IL-27 or anti IL-27 antibodies. RT-PCR from the eyes were performed 6, 12, 24, 48 hours after laser photocoagulation. The CNV was assessed on day 7 by histological examination and image analysis of flat mounts from mice perfused with fluorescein-lableled dextran.
Real-time PCR revealed that the RNA expression of IL-27 was significantly increased after 12hours and maintained for 24hours and 48hours after laser photocoagulation. The mice injected recombinant IL-27 had significantly smaller CNV than control mice. And this inhibitory effect on CNV by IL-27 was denied using anti IL-27 antibody intravitreous injection.
These results suggested that IL-27 has the antiangiogenic activity in the development of laser-induced CNV.
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