April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Haptoglobin Knockout Mice Exhibit a Form of Developmental Glaucoma and Delay of Lens Maturation, Linked to Diminished C-MAF Expression
Author Affiliations & Notes
  • Y. You
    K.U.Leuven, Leuven, Belgium
  • G. Galicias-Rosas
    K.U.Leuven, Leuven, Belgium
  • S. Van de Veire
    K.U.Leuven, Leuven, Belgium
  • T. Van Bergen
    K.U.Leuven, Leuven, Belgium
  • J. Ceuppens
    K.U.Leuven, Leuven, Belgium
  • I. Stalmans
    K.U.Leuven, Leuven, Belgium
  • R. Van Ginderdeuren
    K.U.Leuven, Leuven, Belgium
  • Footnotes
    Commercial Relationships  Y. You, None; G. Galicias-Rosas, None; S. Van de Veire, None; T. Van Bergen, None; J. Ceuppens, None; I. Stalmans, None; R. Van Ginderdeuren, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 865. doi:
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      Y. You, G. Galicias-Rosas, S. Van de Veire, T. Van Bergen, J. Ceuppens, I. Stalmans, R. Van Ginderdeuren; Haptoglobin Knockout Mice Exhibit a Form of Developmental Glaucoma and Delay of Lens Maturation, Linked to Diminished C-MAF Expression. Invest. Ophthalmol. Vis. Sci. 2009;50(13):865.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To study the ocular phenotype of mice that lack Haptoglobin (Hpko) and investigate whether these changes correlate with the expression of C-maf. C-maf is known to be expressed in embryonic life and to be involved in the development of the lens and anterior segment.

Methods: : Hpko mice and wild type(WT)littermates were histological investigated at embryonic day(E)12,15 and 18 and postnatal day(P)1,4 and 21. The expression of C-maf was studied at mRNA level by rt-PCR. C-maf expression in eyes was compared in adult mice between clinically blind versus not-blind Hpko and WT mice and in embryos at E12,between offspring from clinical blind versus not-blind Hpko mothers.

Results: : Eyes of Hpko mice were histological normal at E12 compared with WT mice. At P1 eyes of Hpko mice were significantly smaller (mean 29%); in 55% of mice the lens was not yet separated form the cornea. At P21 12% of the eyes from Hpko mice still showed contact between lens and cornea; anterior senychiae were present in 42% of the eyes, whereas these structural abnormalities were never observed in WT eyes.The ocular C-maf mRNA expression at E12 was significantly lower in Hpko embryos as compared to WT embryos (P<0.05). C-maf expression levels in eyes of Hpko non-blind mothers were intermediate.

Conclusions: : Haptoglobin knockout mice exhibit developmental disorders that are similar to those observed in developmental glaucoma. There is a delay in development of the anterior segment structures, which start during late embryonic life and continue after birth. C-maf expression is diminished at E12 and this may be linked to the aberrant anterior chamber development.

Keywords: anterior segment • genetics • development 

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