April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Influence of Aging on the Secretory and Antioxidant Mechanisms of the Lacrimal Gland
L. M. Tomiyoshi; C. M. Módulo; L. Roma; A. G. Jorge; A. C. Dias; R. Deminice; A. A. Jordão Jr; E. M. Rocha
Author Affiliations & Notes
  • L. M. Tomiyoshi
    Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • C. M. Módulo
    Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • L. Roma
    Departamento de Fisiologia, Universidade Estadual de Campinas, Campinas, Brazil
  • A. G. Jorge
    Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • A. C. Dias
    Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • R. Deminice
    Departamento de Clínica Médica,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • A. A. Jordão Jr
    Departamento de Clínica Médica,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • E. M. Rocha
    Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço,
    Universidade de São Paulo, Ribeirao Preto, Brazil
  • Footnotes
    Commercial Relationships  L.M. Tomiyoshi, None; C.M. Módulo, None; L. Roma, None; A.G. Jorge, None; A.C. Dias, None; R. Deminice, None; A.A. Jordão Jr, None; E.M. Rocha, None.
  • Footnotes
    Support  FAEPA, Fapesp, Cnpq
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 924. doi:
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      L. M. Tomiyoshi, C. M. Módulo, L. Roma, A. G. Jorge, A. C. Dias, R. Deminice, A. A. Jordão Jr, E. M. Rocha; Influence of Aging on the Secretory and Antioxidant Mechanisms of the Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2009;50(13):924.

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Abstract

Purpose: : The objective of the present study was to evaluate the effect of aging on the structure and the secretory mechanisms of the LG.

Methods: : Male Wistar rats aged 24 and 2 months were used. Tears secretion and impression cytology of the cornea were compared. The LG were removed and submitted to structural and morphologic comparison, and analysis of the expression of peroxidase, acetylcholine, vitamin E and MDA. RT PCR was used for the analysis of mRNA expression.

Results: : Impression cytology demonstrated increased metaplasia in the cornea of the aging group (p=0.0320). The Schirmer test was similar in both groups (8.8 ± 1.0 mm for the control and 7.3 ± 1.4 mm for the aging group, p=0.5145). The LG of the aging group showed increased MDA (11.0 ± 0.89 mmol/g and 24.6 ± 1.65 mmol/g, p=0.0571) and peroxidase (1.15 ± 0.52 and 1.05 ± 0.09 mU/mg of LG weight, p=0.34) and reduced vitamin E (4.98 ± 0.50 µM and 1.79 ± 0.18 µM, p=0.0159). The expression of Rab 27b and Rab 3b mRNA was confirmed and decreased with aging (p< 0.05).

Keywords: aging • oxidation/oxidative or free radical damage • lacrimal gland 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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