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S. Usui, K. Komeima, S. Lee, S. Ueno, B. S. Rogers, Z. Wu, J. Shen, L. Lu, B. C. Oveson, P. A. Campochiaro; Co-Expression of Catalase and Superoxide Dismutase 2 in Mitochondria Reduces Cone Cell Death in Retinitis Pigmentosa (RP). Invest. Ophthalmol. Vis. Sci. 2009;50(13):977.
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To test over-expression of components of the endogenous antioxidant defense system in Oxidative damage contributes to cone cell death in RP.
The effect on oxidative damage (ELISA for carbonyl adducts on proteins), cone density, and photopic ERGs was compared in rd1 mice vs. rd1 mice with ubiquitous over-expression of SOD1 (Sod1-rd1 mice), and rd10 mice vs. rd10 mice with inducible expression in the mitochondria of photoreceptors of SOD2 (Sod2-rd10), Catalase (Catalase-rd10), or both (Sod2/Catalase-rd10).
Compared to rd1 mice, Sod1-rd1 mice showed increased retinal carbonyl adducts and decreased photopic b-wave amplitudes at P25, and decreased cone density at P35. Compared to rd10 mice, carbonyl adducts were significantly greater in Sod2-rd10 mice at P35 and significantly less in Sod2/Catalase-rd10 mice at P50.At P50, Sod2/Catalase-rd10 mice had significantly higher photopic b-wave amplitudes and greater cone density than rd10, Sod2-rd10 or Catalase-rd10. There were no differences in scotopic b-wave amplitudes or ONL thickness among the four groups.
Over-expression of SOD1 or 2 alone increase oxidative damage in rd1 or rd10 mice, whereas co-expression of SOD2 and Catalase in mitochondria of photoreceptors significantly reduced oxidative damage and preserved cone cell viability and function after rod cell death.
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