April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Co-Expression of Catalase and Superoxide Dismutase 2 in Mitochondria Reduces Cone Cell Death in Retinitis Pigmentosa (RP)
Author Affiliations & Notes
  • S. Usui
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Department of Ophthalmology, Osaka university school of medicine, Suita, Japan
  • K. Komeima
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • S. Lee
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • S. Ueno
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • B. S. Rogers
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • Z. Wu
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • J. Shen
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • L. Lu
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • B. C. Oveson
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • P. A. Campochiaro
    Departments of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  S. Usui, None; K. Komeima, None; S. Lee, None; S. Ueno, None; B.S. Rogers, None; Z. Wu, None; J. Shen, None; L. Lu, None; B.C. Oveson, None; P.A. Campochiaro, None.
  • Footnotes
    Support  NEI Grant EY05951
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 977. doi:
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      S. Usui, K. Komeima, S. Lee, S. Ueno, B. S. Rogers, Z. Wu, J. Shen, L. Lu, B. C. Oveson, P. A. Campochiaro; Co-Expression of Catalase and Superoxide Dismutase 2 in Mitochondria Reduces Cone Cell Death in Retinitis Pigmentosa (RP). Invest. Ophthalmol. Vis. Sci. 2009;50(13):977.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To test over-expression of components of the endogenous antioxidant defense system in Oxidative damage contributes to cone cell death in RP.

Methods: : The effect on oxidative damage (ELISA for carbonyl adducts on proteins), cone density, and photopic ERGs was compared in rd1 mice vs. rd1 mice with ubiquitous over-expression of SOD1 (Sod1-rd1 mice), and rd10 mice vs. rd10 mice with inducible expression in the mitochondria of photoreceptors of SOD2 (Sod2-rd10), Catalase (Catalase-rd10), or both (Sod2/Catalase-rd10).

Results: : Compared to rd1 mice, Sod1-rd1 mice showed increased retinal carbonyl adducts and decreased photopic b-wave amplitudes at P25, and decreased cone density at P35. Compared to rd10 mice, carbonyl adducts were significantly greater in Sod2-rd10 mice at P35 and significantly less in Sod2/Catalase-rd10 mice at P50.At P50, Sod2/Catalase-rd10 mice had significantly higher photopic b-wave amplitudes and greater cone density than rd10, Sod2-rd10 or Catalase-rd10. There were no differences in scotopic b-wave amplitudes or ONL thickness among the four groups.

Conclusions: : Over-expression of SOD1 or 2 alone increase oxidative damage in rd1 or rd10 mice, whereas co-expression of SOD2 and Catalase in mitochondria of photoreceptors significantly reduced oxidative damage and preserved cone cell viability and function after rod cell death.

Keywords: retinal degenerations: hereditary • oxidation/oxidative or free radical damage • mitochondria 
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