April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Novel Scaffold for Artificial Cornea Prepared by Decellularization of Cornea Using Ultra-high Hydrostatical Pressurization Treatment
S. Sasaki; S. Funamoto; Y. Hashimoto; T. Kimura; T. Honda; S. Hattori; H. Kobayashi; A. Kishida; M. Mochizuki
Author Affiliations & Notes
  • S. Sasaki
    Departments of Ophthalmology, Tokyo Metropolitan Hiroo Hospital, Shibuya-ku Tokyo, Japan
    Department of Ophthalmology, Tokyo Medical and Dental University, Bunkyo-ku Tokyo, Japan
  • S. Funamoto
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku Tokyo, Japan
  • Y. Hashimoto
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku Tokyo, Japan
  • T. Kimura
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku Tokyo, Japan
  • T. Honda
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan
  • S. Hattori
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan
  • H. Kobayashi
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku Tokyo, Japan
    Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki, Japan
  • A. Kishida
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku Tokyo, Japan
  • M. Mochizuki
    Department of Ophthalmology, Tokyo Medical and Dental University, Bunkyo-ku Tokyo, Japan
  • Footnotes
    Commercial Relationships  S. Sasaki, None; S. Funamoto, None; Y. Hashimoto, None; T. Kimura, None; T. Honda, None; S. Hattori, None; H. Kobayashi, None; A. Kishida, None; M. Mochizuki, None.
  • Footnotes
    Support  Grants-in-Aid 1700503
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1509. doi:
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      S. Sasaki, S. Funamoto, Y. Hashimoto, T. Kimura, T. Honda, S. Hattori, H. Kobayashi, A. Kishida, M. Mochizuki; Novel Scaffold for Artificial Cornea Prepared by Decellularization of Cornea Using Ultra-high Hydrostatical Pressurization Treatment. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1509.

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Abstract

Purpose: : To evaluate the biocompatibility of corneal stroma prepared by decellularization of cornea using ultra-high hydrostatical pressurization (UHP) treatment.

Methods: : Porcine corneal stroma was decellularized by the ultra-high hydrostatical pressure method. Hematoxylin-eosin (H-E) staining was performed to confirm the removal of the corneal cells. Then a 6-mm-diameter decellularized porcine corneal stromal disc was implanted into rabbit corneal pocket with a 2-mm-diameter window. Six months later, the rabbit eye was enucleated to examine the inflammation and the progress of the rabbit epithelium over the porcine corneal stromal disc.

Results: : Complete removal of cells was confirmed by histopathological examination in UHP-decellularized corneal stroma. Only little inflammation was observed when UHP-decellularized porcine corneal stroma was implanted into rabbit corneal pocket. Surprisingly, it was covered with rabbit corneal epithelium.

Conclusions: : UHP-decellularized corneal stroma has extremely high biocompatibility and is a possible candidate of corneal scaffold for artificial cornea.

Keywords: cornea: stroma and keratocytes • transplantation 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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