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S. Sasaki, S. Funamoto, Y. Hashimoto, T. Kimura, T. Honda, S. Hattori, H. Kobayashi, A. Kishida, M. Mochizuki; Novel Scaffold for Artificial Cornea Prepared by Decellularization of Cornea Using Ultra-high Hydrostatical Pressurization Treatment. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1509.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the biocompatibility of corneal stroma prepared by decellularization of cornea using ultra-high hydrostatical pressurization (UHP) treatment.
Porcine corneal stroma was decellularized by the ultra-high hydrostatical pressure method. Hematoxylin-eosin (H-E) staining was performed to confirm the removal of the corneal cells. Then a 6-mm-diameter decellularized porcine corneal stromal disc was implanted into rabbit corneal pocket with a 2-mm-diameter window. Six months later, the rabbit eye was enucleated to examine the inflammation and the progress of the rabbit epithelium over the porcine corneal stromal disc.
Complete removal of cells was confirmed by histopathological examination in UHP-decellularized corneal stroma. Only little inflammation was observed when UHP-decellularized porcine corneal stroma was implanted into rabbit corneal pocket. Surprisingly, it was covered with rabbit corneal epithelium.
UHP-decellularized corneal stroma has extremely high biocompatibility and is a possible candidate of corneal scaffold for artificial cornea.
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