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G. Modorati, E. Miserocchi, C. Fortis, P. Scarpellini, A. Colucci, P. Rama; Enzyme-Linked Immunospot (ELISPOT)-Interferon Gamma Assay and Tuberculin Skin Test (TST) in Patients With Uveitis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1546. doi: https://doi.org/.
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to evaluate an Enzyme-Linked Immunospot (ELISPOT)-Interferon gamma assay and tuberculin skin test (TST) in detecting latent Mycobacterium tuberculosis infection in patients with uveitis.
a total of 43 patients with uveitis followed at the Ocular Immunology Service of the San Raffaele University Hospital-Milan, underwent the Mantoux TST and ELISPOT-IFN-gamma assay. All diagnostic tests were performed in the laboratory of the Infectious Disease Department of the San Raffaele Hospital. Ocular diagnosis were grouped in: serpiginous choroiditis (9 pts), posterior uveitis (10 pts), panuveitis (14 pts) and anterior segment inflammatory diseases (uveitis and scleritis) (10 pts). Patients characteristics evaluated included: demographic data (age, gender, race), ocular data (laterality, visual acuity).
43 caucasian patients (20 Female, 23 Male), with a median age of 53 years (range 9-85) were evaluated. Ocular involvement was bilateral in 76% of patients. The median visual acuity at the time of testing was 0,7 (range: 0.02-1.0). The rate of positivity in the whole population was: 73% for TST and 58% for ELISPOT. In different subset of diagnosis TST and ELISPOT positivity were respectively: 89% and 78% in the serpiginous choroiditis group; 80% and 70% in the posterior uveitis group; 57% and 43% in the panuveitis group; 80% and 45% in the anterior segment inflammation group.
ELISPOT has shown to be more accurate than TST in identifying subjects with latent TB infection. This diagnostic test could improve detection of uveitis of tuberculous etiology.
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