April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Enzyme-Linked Immunospot (ELISPOT)-Interferon Gamma Assay and Tuberculin Skin Test (TST) in Patients With Uveitis
Author Affiliations & Notes
  • G. Modorati
    Dept of Ophthalmology,
    Univ Hospital San Raffaele, Milan, Italy
  • E. Miserocchi
    Dept of Ophthalmology,
    Univ Hospital San Raffaele, Milan, Italy
  • C. Fortis
    Laboratory of Clinical Immunology, Division of Immunology and Transplant,
    Univ Hospital San Raffaele, Milan, Italy
  • P. Scarpellini
    Infectious Diseases Department,
    Univ Hospital San Raffaele, Milan, Italy
  • A. Colucci
    Dept of Ophthalmology,
    Univ Hospital San Raffaele, Milan, Italy
  • P. Rama
    Dept of Ophthalmology,
    Univ Hospital San Raffaele, Milan, Italy
  • Footnotes
    Commercial Relationships  G. Modorati, None; E. Miserocchi, None; C. Fortis, None; P. Scarpellini, None; A. Colucci, None; P. Rama, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1546. doi:https://doi.org/
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      G. Modorati, E. Miserocchi, C. Fortis, P. Scarpellini, A. Colucci, P. Rama; Enzyme-Linked Immunospot (ELISPOT)-Interferon Gamma Assay and Tuberculin Skin Test (TST) in Patients With Uveitis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1546. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : to evaluate an Enzyme-Linked Immunospot (ELISPOT)-Interferon gamma assay and tuberculin skin test (TST) in detecting latent Mycobacterium tuberculosis infection in patients with uveitis.

Methods: : a total of 43 patients with uveitis followed at the Ocular Immunology Service of the San Raffaele University Hospital-Milan, underwent the Mantoux TST and ELISPOT-IFN-gamma assay. All diagnostic tests were performed in the laboratory of the Infectious Disease Department of the San Raffaele Hospital. Ocular diagnosis were grouped in: serpiginous choroiditis (9 pts), posterior uveitis (10 pts), panuveitis (14 pts) and anterior segment inflammatory diseases (uveitis and scleritis) (10 pts). Patients characteristics evaluated included: demographic data (age, gender, race), ocular data (laterality, visual acuity).

Results: : 43 caucasian patients (20 Female, 23 Male), with a median age of 53 years (range 9-85) were evaluated. Ocular involvement was bilateral in 76% of patients. The median visual acuity at the time of testing was 0,7 (range: 0.02-1.0). The rate of positivity in the whole population was: 73% for TST and 58% for ELISPOT. In different subset of diagnosis TST and ELISPOT positivity were respectively: 89% and 78% in the serpiginous choroiditis group; 80% and 70% in the posterior uveitis group; 57% and 43% in the panuveitis group; 80% and 45% in the anterior segment inflammation group.

Conclusions: : ELISPOT has shown to be more accurate than TST in identifying subjects with latent TB infection. This diagnostic test could improve detection of uveitis of tuberculous etiology.

Keywords: microbial pathogenesis: clinical studies • clinical laboratory testing • bacterial disease 
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