April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Matrix Metalloproteinase (MMP)-9 Is Expressed During Corneal Perforation in Chronic Graft-versus-Host Disease
E. Inagaki; Y. Ogawa; Y. Matsumoto; T. Kawakita; S. Shimmura; K. Tsubota
Author Affiliations & Notes
  • E. Inagaki
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • Y. Ogawa
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • Y. Matsumoto
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • T. Kawakita
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • S. Shimmura
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • K. Tsubota
    Ophthalmology, Keio University School of Medicine, Tokyo city, Japan
  • Footnotes
    Commercial Relationships  E. Inagaki, None; Y. Ogawa, None; Y. Matsumoto, None; T. Kawakita, None; S. Shimmura, None; K. Tsubota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1747. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Matrix Metalloproteinase (MMP)-9 Is Expressed During Corneal Perforation in Chronic Graft-versus-Host Disease
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      E. Inagaki, Y. Ogawa, Y. Matsumoto, T. Kawakita, S. Shimmura, K. Tsubota; Matrix Metalloproteinase (MMP)-9 Is Expressed During Corneal Perforation in Chronic Graft-versus-Host Disease. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1747.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To investigate the etiology of corneal perforation in human ocular chronic graft-versus-host disease (cGVHD) following allogenic hematopoietic stem cell transplantation.

Methods: : Four ocular cGVHD patients (1 female, 3 males; mean age 52.3±21.6 years) suffering from corneal perforation were enrolled in the study. After informed consents were obtained, we examined perforated corneal samples obtained following keratoplasty. Paraffin-embedded and frozen tissue sections were examined by hematoxin-eosin staining and immunohistochemistry. Eye Bank donor corneas were used as controls.

Results: : Hematoxin-eosin staining showed that neutrophils were scarcely detected around the perforated ulcerative lesion in cGVHD corneas. However, immunohistochemistry revealed that the number of CD68-positive macrophages surrounding the perforated lesion increased compared to control. Both CD4+ and CD8+ T cells were not detected at the site of corneal perforation in this study. Immunoreactivity for matrix metalloproteinase (MMP)-9 was detected in the corneal stroma and endothelium, but not in control samples. MMP-2 was not detected both cGVHD and control samples.

Keywords: cornea: basic science • immunohistochemistry • transplantation 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept