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M. Lopez, A. de la Mata, S. Galindo, V. Sáez, T. Nieto-Miguel, O. Riera, M. de la Paz, J. M. Herreras, M. Calonge, R. M. Corrales; Repeated Expansion of Limbal Stem Cells From a Single Biopsy. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1787.
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Transplantation of autologous ex vivo expanded limbal epithelial stem cells (LESCs) is becoming the method of choice to reconstruct the ocular surface affected by LESC deficiency. This can be hampered by the small amount of tissue available and the possibility of failure in the cell expansion procedure. We investigated the feasibility to obtain more than one expanded sheet of cells from the same limbal biopsy.
We obtained up to LPC7 with p-FBS and pm-FBS. pm-HS and Cnt20 allowed to obtain up to LPC3. The fastest cell growth was observed with p-FBS. With p-FBS, K3 and K7 expression increased (K3: 73-85%; K 7(48-61%) from LPC1 to LPC3, respectively; K5 (50%) and K19 (46%) did not change and K14 (50-43%) and K15 (48%-13%) decreased until LPC3. With pm-HS, all markers increased from LPC1 to LPC3 (K5: 4-48%, K14: 3-35%, respectively) except K3 and K15. The profile obtained with pm-FBS was not adequate (0% for K5, K14, K15). With Cnt20 all markers decreased in LPC3, except K3.
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