April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Analysis of the Effects of SLC4A11 (CHED2) Gene Depletion on Corneal Endothelial Cell Growth and Proliferation
E. N. Vithana; A. Venkatraman; L. Koh; R. R. Mohan; L. Seet
Author Affiliations & Notes
  • E. N. Vithana
    Singapore Eye Rsch Institute, Singapore, Singapore
    Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
  • A. Venkatraman
    Singapore Eye Rsch Institute, Singapore, Singapore
  • L. Koh
    Singapore Eye Rsch Institute, Singapore, Singapore
  • R. R. Mohan
    Mason Eye Institute and College of Veterinary Medicine, University of Missouri-Columbia, Columbia, Missouri
  • L. Seet
    Singapore Eye Rsch Institute, Singapore, Singapore
  • Footnotes
    Commercial Relationships  E.N. Vithana, None; A. Venkatraman, None; L. Koh, None; R.R. Mohan, None; L. Seet, None.
  • Footnotes
    Support  Biomedical Research Council (Singapore) Grant 07/1/35/19/520
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1799. doi:
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      E. N. Vithana, A. Venkatraman, L. Koh, R. R. Mohan, L. Seet; Analysis of the Effects of SLC4A11 (CHED2) Gene Depletion on Corneal Endothelial Cell Growth and Proliferation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1799.

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Abstract

Results: : - SLC4A11 gene was successfully depleted at 10nM concentration by the two siRNAs at mRNA level. The HA-tagged SLC4A11 protein produced from a plasmid co-transfected with siRNA was also shown to be depleted by western analysis. The siRNA treatment for 48, 72 or 96 h appeared to have a stimulatory effect on cell proliferation and growth, although mRNA levels showed >95% depletion at all time points, compared to cells treated with negative siRNA control. SLC4A11 depletion also reduced the expression of pro-apoptotic factor Bax by about 50% and produced a negative result on TUNEL assay. Borate concentrations (1uM-1mM) were found not to significantly induce CEC growth compared to untreated cells. Concentrations 5-10mM were found to be slightly toxic to CECs.Conclusion- The effect of SLC4A11 depletion in transformed corneal endothelial cells differs from that observed in HeLa cells, where transient gene depletion reduced cell growth and proliferation. Work is in progress to test the effects of stable knockdown of SLC4A11. It is possible that NaBC1 has a different function in CECs and needs further investigation.

Keywords: cornea: endothelium • proteins encoded by disease genes • gene/expression 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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