April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Cultivated Monkey Corneal Endothelial Cell Transplantation Using Corneal Lamellar Graft
N. Koizumi; N. Okumura; Y. Sakamoto; H. Takahashi; K. Hirata; N. Okahara; H. Tsuchiya; R. Torii; T. Inatomi; S. Kinoshita
Author Affiliations & Notes
  • N. Koizumi
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • N. Okumura
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Y. Sakamoto
    Research Laboratory, Senju Pharmaceutical Co., Ltd., Kobe, Japan
  • H. Takahashi
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • K. Hirata
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • N. Okahara
    Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Japan
  • H. Tsuchiya
    Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Japan
  • R. Torii
    Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Japan
  • T. Inatomi
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships  N. Koizumi, None; N. Okumura, None; Y. Sakamoto, None; H. Takahashi, None; K. Hirata, None; N. Okahara, None; H. Tsuchiya, None; R. Torii, None; T. Inatomi, None; S. Kinoshita, None.
  • Footnotes
    Support  the Academic Frontier Research Project on the New Frontier of Biomedical Engineering Research and a Grant-in Aid for Scientific Research 16791076 from the Japanese Ministry of ECSST
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1811. doi:
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      N. Koizumi, N. Okumura, Y. Sakamoto, H. Takahashi, K. Hirata, N. Okahara, H. Tsuchiya, R. Torii, T. Inatomi, S. Kinoshita; Cultivated Monkey Corneal Endothelial Cell Transplantation Using Corneal Lamellar Graft. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1811.

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Abstract

Purpose: : Previously, we established a primate animal model of cultivated corneal endothelial transplantation using cynomolgous monkeys whose corneal endothelial cells have low proliferative ability (Koizumi, et al. IOVS 2007). The present study was designed to investigate the feasibility of cultivated corneal endothelial cell transplantation using a corneal lamellar graft in animal eyes with corneal endothelial dysfunction.

Methods: : The experimental protocol was approved by the regulations of university animal care and use committees, and animals were handled in accordance with ARVO guidelines. Descemet’s membrane with intact corneal endothelial cells was removed from human corneal eye bank tissue. Corneal lamellar grafts (150µm thickness and 8mm diameter) were made from the posterior stroma with a Moria microkeratome. Cultivated monkey corneal endothelial cells (MCECs) were seeded on the human corneal lamellar grafts and cultivated for 3 weeks. Under general anesthesia, the corneal endothelia and Descemet’s membrane of 2 eyes of 2 animals (1 rabbit and 1 monkey) were removed, and the grafts with MCECs were transplanted using Busin glide in a similar procedure to Descemet-stripping automated endothelial keratoplasty (DSAEK).

Results: : Confluent MCECs were established on the graft with a density of 2200 cells/mm2 and expressed ZO-1 and Na+-K+ ATPase. The day after surgery, grafts were well attached to the host corneal stroma and mild corneal edema was observed. Histological examination of the rabbit eye 2 days after surgery showed no donor endothelial damage due to the graft insertion. The monkey cornea recovered its clarity by 2 weeks postoperatively. Two months after surgery, the monkey cornea remained clear, was 750µm thick, with hexagonal cells observed by specular microscopy at a density of 2200 cells/mm2. No signs of rejection were detected.

Conclusions: : We cultivated MCECs on a human corneal lamellar graft and successfully transplanted this construct into animal eyes with the DSAEK technique. Our results suggest the possibility of cultivated corneal endothelial cell transplantation using a corneal lamellar graft.

Keywords: cornea: endothelium • transplantation 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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