April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
In vitro and ex vivo Effects of FGF-2 on Cell Proliferation and p27 Expression in Human Corneal Endothelial Cells
J.-S. Song; J. Lee; R. E. Smith; E. P. Kay
Author Affiliations & Notes
  • J.-S. Song
    Doheny Eye Institute, Los Angeles, California
    Ophthalmology, Korea University College of Medicine, Seoul, Republic of Korea
  • J. Lee
    Doheny Eye Institute, Los Angeles, California
  • R. E. Smith
    Doheny Eye Institute, Los Angeles, California
    Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • E. P. Kay
    Doheny Eye Institute, Los Angeles, California
    Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  J.-S. Song, None; J. Lee, None; R.E. Smith, None; E.P. Kay, None.
  • Footnotes
    Support  NIH/NEI EY06431 and EY03040, and Research to Prevent Blindness, New York, NY
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1812. doi:
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      J.-S. Song, J. Lee, R. E. Smith, E. P. Kay; In vitro and ex vivo Effects of FGF-2 on Cell Proliferation and p27 Expression in Human Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1812.

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Abstract

Purpose: : Fibroblast growth factor 2 (FGF-2) is known to stimulate cell proliferation of rabbit corneal endothelial cells (CECs) by degrading p27. We investigated the effect of FGF-2 on cell proliferation and p27 expression in human CECs in ex vivo and in vitro studies.

Methods: : hCECs were isolated with EDTA treatment; cell proliferation was determined by MTT assay. Pharmacological inhibitors were used to block PI 3-kinase and ERK1/2. Subcellular localization of p27 and phosphorylated p27 (pp27) was determined using immunofluorescent staining.

Results: : : FGF-2 stimulated cell proliferation in hCECs and these effects were inhibited by LY294002 and U0126. Cells maintained in mitogen-deprived medium were stained for p27 in the nuclei, but not for pp27. However, FGF-2 abolished the staining of p27, while nuclear staining of pp27 was observed. Such effect of FGF-2 on p27 and pp27 expression was blocked with LY294002. Likewise, the ex vivo human corneal endothelium showed nuclear p27 staining in mitogen-deprived medium. However, p27 expression was significantly decreased with FGF-2 treatment.

Conclusions: : FGF-2 stimulates cell proliferation in hCECs through PI-3 kinase and ERK 1/2 pathways and significantly downregulates p27 through phosphorylation mechanism, as observed in rabbit CECs.

Keywords: cornea: endothelium 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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