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H. Takahashi, N. Koizumi, N. Okumura, Y. Sakamoto, K. Hirata, M. Yamamoto, Y. Tsukahara, T. Isobe, Y. Tabata, S. Kinoshita; Corneal Endothelial Wound Healing Is Promoted by the Injection of bFGF-Impregnated Gelatin Microspheres in Rabbit Cryo-Injury Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1827.
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To determine the effect of the injection of bFGF-impregnated gelatin microspheres (bFGF-GMS) into the anterior chamber on a rabbit corneal endothelial cryo-injury model.
In 5 Japanese white rabbits under general anesthesia, the central corneal endothelium was damaged by transcorneal freezing with a 7-mm-diameter probe tip. The probes were applied for 15 seconds. In addition, the right eye of each rabbit was injected with 30 ng of bFGF/0.2 mg GMS (bFGF-GMS group) into the anterior chamber. The left eyes were injected with 0.2 mg of GMS only (GMS group). On the third day after injection, anterior segment examination with a slit-lamp biomicroscope was performed, and intraocular pressure (IOP) was then measured. All animals were euthanized and the aqueous humor and corneal tissue were then collected. The concentration of bFGF in the aqueous humor was determined by ELISA. Each cornea was stained with Alizarin red to evaluate the endothelial denuded area and immunostained for Ki67.
The denuded area of the bFGF-GMS group was decreased significantly as compared with the GMS group (15% vs. 100%, respectively) (P<0.01; Student’s t-test). At the border of wound, positive staining of the proliferative marker Ki67 was observed. Forty-eight hours after the injection, bFGF concentration in the aqueous humor was 22 pg/mL. IOP elevation and neovascularization were not observed at the applied dose.
A single injection of bFGF-GMS into the anterior chamber accelerates wound closure in a rabbit endothelial injury model without IOP elevation or neovascularization. These results suggest that the sustained release of bFGF is best suited to corneal endothelial wound healing in vivo. Though long-term observations are necessary, our results provide useful information for the possible future clinical application of bFGF-GMS in tackling corneal endothelial dysfunction.
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