April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
RPE Wound Healing Induces Upregulation of Laminin 5
S. He; S. Kase; J. Xu; M. Kitamura; C. Kim; S. Ryan; D. Hinton
Author Affiliations & Notes
  • S. He
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
    Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • S. Kase
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
  • J. Xu
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
  • M. Kitamura
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
  • C. Kim
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
  • S. Ryan
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
  • D. Hinton
    Ophthalmology-USC, Doheny Eye Institute, Los Angeles, California
    Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  S. He, None; S. Kase, None; J. Xu, None; M. Kitamura, None; C. Kim, None; S. Ryan, None; D. Hinton, None.
  • Footnotes
    Support  NIH grants EY 02061, EY 03040 & grants from RPB & the Arnold & Mabel Beckman foundation
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1840. doi:
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      S. He, S. Kase, J. Xu, M. Kitamura, C. Kim, S. Ryan, D. Hinton; RPE Wound Healing Induces Upregulation of Laminin 5. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1840.

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Abstract

Purpose: : Extracellular matrix (ECM) production plays an important role in the wound healing response. The purpose of this study was to investigate the expression of Laminin 5 in a wound healing model using human retinal pigment epithelial cells (RPE) and the effects of laminin 5 on RPE cell function.

Methods: : Confluent cultures of early passage human fetal RPE cells were scratched with a pipette tip and Laminin 5 expression in the wounded area was demonstrated by immunofluorescent staining and western blot at different time points after wounding. Laminin 5 mRNA expression in RPE cells was revealed by RT-PCR. Laminin 5 effects on RPE cell attachment and proliferation were evaluated using a modified MTT assay.

Results: : Cultured human RPE cells express Laminin 5 mRNA and protein. Wounding of the RPE monolayer resulted in an abundant increase of Laminin 5 at 16-24 hours after scratch as demonstrated by immunofluorescent staining and western blot. RPE cell attachment was reduced by 50% at 5 minutes and 30% at 10 minutes (P<0.01); RPE proliferation was inhibited by 30% at the concentration of 1 ug/ml Laminin 5 (p<0.05).

Conclusions: : RPE cell attachment and proliferation were significantly inhibited by treatment with recombinant Laminin 5.Conclusions: Laminin 5 is upregulated in response to wound healing in RPE cells. Recombinant Laminin 5 inhibits RPE attachment and proliferation.

Keywords: proliferative vitreoretinopathy • retina • wound healing 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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