April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Cross-Talk Between Vascular Endothelial Growth Factor and Calcium-Independent Phospholipase A2 in the Regulation of Retinal Pigment Epithelium Cell Migration and Proliferation
Author Affiliations & Notes
  • E. C. Andersen
    Department of Neuroscience and Pharmacology, Section of Eye Pathology,
    Department of International Health, Immunology and Microbiology, Immunology and Ocular Biology,
    University of Copenhagen, Copenhagen, Denmark
  • J. R. Andreasen
    Department of Neuroscience and Pharmacology, Section of Eye Pathology,
    Department of International Health, Immunology and Microbiology, Immunology and Ocular Biology,
    University of Copenhagen, Copenhagen, Denmark
  • K. A. Poulsen
    Department of Biology, Section of Cell- and Developmental Biology,
    University of Copenhagen, Copenhagen, Denmark
  • N. Junker
    Institute of Biomedical Sciences, The Faculty of Health Sciences,
    University of Copenhagen, Copenhagen, Denmark
  • M. H. Nissen
    Department of International Health, Immunology and Microbiology, Immunology and Ocular Biology,
    University of Copenhagen, Copenhagen, Denmark
  • M. Kolko
    Department of Neuroscience and Pharmacology, Section of Eye Pathology,
    Department of International Health, Immunology and Microbiology, Immunology and Ocular Biology,
    University of Copenhagen, Copenhagen, Denmark
  • Footnotes
    Commercial Relationships  E.C. Andersen, None; J.R. Andreasen, None; K.A. Poulsen, None; N. Junker, None; M.H. Nissen, None; M. Kolko, None.
  • Footnotes
    Support  Synoptik-Fonden
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1853. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. C. Andersen, J. R. Andreasen, K. A. Poulsen, N. Junker, M. H. Nissen, M. Kolko; Cross-Talk Between Vascular Endothelial Growth Factor and Calcium-Independent Phospholipase A2 in the Regulation of Retinal Pigment Epithelium Cell Migration and Proliferation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1853.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : During pathological conditions the retinal pigment epithelium (RPE) cells can be triggered to proliferate and migrate.Vascular endothelial growth factor (VEGF) and calcium-independent phospholipase A2, group IVA (iPLA2-VIA) have been shown to be involved in cell proliferation and migration1,2. The purpose of this study was to evaluate the role of iPLA2-VIA in migration of RPE cells and to investigate a possible cross talk between VEGF and iPLA2-VIA.

Methods: : ARPE-19 cells were transfected with an iPLA2-VIA promoter-luciferase constructs to elucidate the regulation of iPLA2-VIA after exposure to VEGF. Western blot analysis was performed to investigate the expression of VEGF in cells transfected with an iPLA2-VIA construct. Proliferation of ARPE-19 cells was evaluated by incorporation of radioactively labeled thymidine and migration was evaluated by the use of a Boyden chamber. VEGF was added to cells and effect of iPLA2-VIA was examined by exposing the cells for commercial iPLA2 inhibitors.

Results: : The present study reveal an upregulation of the promoter gene for iPLA2-VIA as well as an upregulation of iPLA2-VIA-protein in ARPE-19 cells treated with VEGF. Exposure of VEGF stimulates ARPE-19 cells to proliferate and migrate. Both proliferation and migration can be reduced by iPLA2-inhibitors.

Conclusions: : The present results show a cross talk between VEGF and iPLA2-VIA and indicate a role of iPLA2-VIA in the regulation of RPE proliferation and migration.

Keywords: retinal pigment epithelium • vascular endothelial growth factor • proliferation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×