April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
LYVE-1 Is Expressed During the Development and Regression of the Hyaloid Vascular System
H. Zhang; J. Tse; X. Hu; J. Kang; F. Tilahun; Y. Hong; M. Qiu; L. Chen
Author Affiliations & Notes
  • H. Zhang
    Center for Eye Disease and Development, Program in Vision Science, School of Opthometry, UC at Berkeley, University of California at Berkeley, California
  • J. Tse
    Center for Eye Disease and Development, Program in Vision Science, School of Opthometry, UC at Berkeley, University of California at Berkeley, California
  • X. Hu
    Department of Anatomical Sciences & Neurobiology, University of Louisville, School of Medicine, Louisville, Kentucky
  • J. Kang
    Departments of Surgery and Biochemistry & Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California
  • F. Tilahun
    Center for Eye Disease and Development, Program in Vision Science, School of Opthometry, UC at Berkeley, University of California at Berkeley, California
  • Y. Hong
    Departments of Surgery and Biochemistry & Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California
  • M. Qiu
    Department of Anatomical Sciences & Neurobiology, University of Louisville, School of Medicine, Louisville, Kentucky
  • L. Chen
    Center for Eye Disease and Development, Program in Vision Science, School of Opthometry, UC at Berkeley, University of California at Berkeley, California
  • Footnotes
    Commercial Relationships  H. Zhang, None; J. Tse, None; X. Hu, None; J. Kang, None; F. Tilahun, None; Y. Hong, None; M. Qiu, None; L. Chen, None.
  • Footnotes
    Support  NIH grant EY17392, DoD grant and Junior Faculty Research Grant from University of California at Berkeley (LC)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1953. doi:
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      H. Zhang, J. Tse, X. Hu, J. Kang, F. Tilahun, Y. Hong, M. Qiu, L. Chen; LYVE-1 Is Expressed During the Development and Regression of the Hyaloid Vascular System. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1953.

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Abstract

Purpose: : The hyaloid vascular system (HVS), which is thought to consist of only arteriesis, is a transient network nourishing the embryonic lens and the primary vitreous of the developing eye. Failure of complete regression of this hyaloid vasculature is associated with several blinding ocular pathologies including hyperplastic primary vitreous and persistent fetal vasculature. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a newly discovered lymphatic marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the hyaloid vascular system. The purpose of this study was to investigate the expression of LYVE-1 during the development and regression of the hyaloid vascular system.

Methods: : Normal C57BL/6 mouse eyeballs were sampled from embryonic day (E)10.5 to postnatal (P) stages and cryosections were investigated by light, deconvolutional fluorescent, and confocal microscopic studies using LYVE-1, podoplanin, and CD31 antibodies. Additionally, these sections were also stained for CD45, CD11b, and F4/80.

Results: : LYVE-1 positive cells were detected in the tunica vasculosa lentis (TVL) and vasa hyaloidea propria (VHP) in the mouse eyes between E13.5~14.5 to P14~16. These cells are CD31 and podoplanin negative but CD45, CD11b, and F4/80 positive, indicating a macrophage lineage.

Keywords: neovascularization • development • microscopy: light/fluorescence/immunohistochemistry 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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