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H. Zhang, J. Tse, X. Hu, J. Kang, F. Tilahun, Y. Hong, M. Qiu, L. Chen; LYVE-1 Is Expressed During the Development and Regression of the Hyaloid Vascular System. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1953.
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© ARVO (1962-2015); The Authors (2016-present)
The hyaloid vascular system (HVS), which is thought to consist of only arteriesis, is a transient network nourishing the embryonic lens and the primary vitreous of the developing eye. Failure of complete regression of this hyaloid vasculature is associated with several blinding ocular pathologies including hyperplastic primary vitreous and persistent fetal vasculature. Lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) is a newly discovered lymphatic marker that is also expressed by a subpopulation of macrophages. To date, there is no report on its expression in the hyaloid vascular system. The purpose of this study was to investigate the expression of LYVE-1 during the development and regression of the hyaloid vascular system.
Normal C57BL/6 mouse eyeballs were sampled from embryonic day (E)10.5 to postnatal (P) stages and cryosections were investigated by light, deconvolutional fluorescent, and confocal microscopic studies using LYVE-1, podoplanin, and CD31 antibodies. Additionally, these sections were also stained for CD45, CD11b, and F4/80.
LYVE-1 positive cells were detected in the tunica vasculosa lentis (TVL) and vasa hyaloidea propria (VHP) in the mouse eyes between E13.5~14.5 to P14~16. These cells are CD31 and podoplanin negative but CD45, CD11b, and F4/80 positive, indicating a macrophage lineage.
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