April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Corneal Antigen Presenting Cells Promote Allograft Survival
Author Affiliations & Notes
  • J. Schwartzkopff
    University Eye Hospital, Freiburg, Germany
  • D. R. Saban
    Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts
  • A. Beiter
    University Eye Hospital, Freiburg, Germany
  • I. T. Gross
    University Eye Hospital, Freiburg, Germany
  • F. Birnbaum
    University Eye Hospital, Freiburg, Germany
  • D. Böhringer
    University Eye Hospital, Freiburg, Germany
  • T. Reinhard
    University Eye Hospital, Freiburg, Germany
  • Footnotes
    Commercial Relationships  J. Schwartzkopff, None; D.R. Saban, None; A. Beiter, None; I.T. Gross, None; F. Birnbaum, None; D. Böhringer, None; T. Reinhard, None.
  • Footnotes
    Support  Ernst-und-Berta Grimmke Foundation, Düsseldorf, Germany
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 1964. doi:https://doi.org/
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    • Get Citation

      J. Schwartzkopff, D. R. Saban, A. Beiter, I. T. Gross, F. Birnbaum, D. Böhringer, T. Reinhard; Corneal Antigen Presenting Cells Promote Allograft Survival. Invest. Ophthalmol. Vis. Sci. 2009;50(13):1964. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The risk of corneal allograft rejection seen clinically in children and in infants is considerably high. Baby rat recipients have previously been described as a good model to study this, as they indeed demonstrate accelerated rejection. In this set of experiments a potential difference in the immunogenicity of a corneal transplant depending on the donor age was analyzed in the baby rat model.

Methods: : Corneal allografts were harvested from baby or adult Fisher rats (3 or 10 weeks old respectively) and orthotopically placed on Lewis rats. In addition chimeric corneal allografts consisting of stroma-endothelium from adult Fisher rats covered by corneal epithelium from baby Lewis rats (or reciprocal chimeric grafts) were also placed orthotopically on baby Lewis rats. Graft opacity was regularly assessed until rejection and immunohistological analyses of corneal antigen presenting (APC) cells were performed by fluorescence and confocal microscopy.

Results: : In contrast to normal adult corneas, the center of normal baby corneas from donor Fisher rats had large numbers of APCs (DC, CD11c, MHC-II, CD163) in the epithelial layer. Nonetheless, rejection of such baby allografts was markedly decreased in baby recipients (p<0.01), and similarly observed in adult recipients as well (p<0.01). Interestingly, chimeric allografts bearing baby corneal epithelium covering adult stroma-endothelium, showed a strong decrease in the rejection rate (p<0.01) compared to reciprocal chimeric allografts.

Conclusions: : The immunogenicity of baby corneal allografts appears to be markedly reduced relative to adult donors. Furthermore, our data suggest that this is due to donor corneal APC born by baby corneal epithelium. We therefore demonstrate evidence that donor corneal APC can promote corneal graft survival.

Keywords: cornea: epithelium • cornea: basic science • immune tolerance/privilege 
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