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J. Liang, M. J. Hanson, S. Song; Protein-Protein Interaction of Lens S-Crystallin and Its Congenital Cataract G18V Mutant. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2104.
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© ARVO (1962-2015); The Authors (2016-present)
γS-crystallin is one of the lens γ-crystallins and differs in developmental expression pattern from other γ-crystallins. It is expressed in late stage as opposed to other γ-crystallins being early genes. Thus, γS-crystallin is distributed in the cortical region. We have investigated its interaction properties with other γ-crystallins as well as with the congenital cataract G18V mutant.
A two-hybrid system assay was used to detect protein-protein interactions. γS-crystallin and its congenital cataract mutant G18V were expressed and their biophysical properties, such as secondary and tertiary structure, thermal and conformational stability, and hydrophobicity, were compared.
γS-crystallin shows some protein-protein interaction with other γ-crystallins (γC- and γD-) and some enhancement of interaction with G18V γS-crystallin mutant. The mutant shows no conformational change, but shows decreased thermal and conformational stability as well as increased hydrophobicity.
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