April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Bleaching of Age-Related Lens Yellowing by Two-Photon Photolysis
Author Affiliations & Notes
  • L. Kessel
    Ophthalmology, Glostrup Hospital, Glostrup, Denmark
  • L. Eskildsen
    Ophthalmology, Glostrup Hospital, Glostrup, Denmark
  • M. van der Poel
    Department of Photonics Engineering, Technical University of Denmark, Lyngby, Denmark
  • M. Larsen
    Ophthalmology, Glostrup Hospital, Glostrup, Denmark
  • Footnotes
    Commercial Relationships  L. Kessel, None; L. Eskildsen, None; M. van der Poel, None; M. Larsen, Inventor of patent, P.
  • Footnotes
    Support  Danish National Advanced Technology Foundation and the Danish Medical Research Council (grant # 721-05-0689)
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2118. doi:
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      L. Kessel, L. Eskildsen, M. van der Poel, M. Larsen; Bleaching of Age-Related Lens Yellowing by Two-Photon Photolysis. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2118.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : The aged human lens contains chromophores that absorb and scatter incoming light to an extent which may interfere with visual function. The aim of the present study was to produce photochemical bleaching of the age-related lens yellowing by two-photon photolysis.

Methods: : Ten human donor lenses were exposed to ultrashort infrared laser pulses from a femtosecond Ti:Sapphire laser emitting at 800 nm. Age range of the donors was 46 to 75 years of age. Pulse duration was 200-300 femtoseconds. Pulse energy was approximately 0.5 µJ. A focusing lens of f=85 mm was used yielding a theoretical spotsize of 14 µm.

Results: : Two-photon absorption was evident as emission of blue autofluorescence upon exitation by pulsed infrared light at 800 nm. Photobleaching was observed as an increased transmission of light in the blue-green range of the spectrum and as a macroscopically visible reduced yellow coloration of the aged human donor lenses whilst lens transparency was maintained.

Conclusions: : We found that exposure to ultrashort pulsed infra-red laser light lead to bleaching the chromophores through twophoton absorption. This leaves hopes for a new non-invasive treatment of age-related lens disorders.

Keywords: cataract • laser • crystallins 

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