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M. Wu, R. Peng, Y. Zhao, Z. Liu, X. Liu, F. Shang; Expression of Dominant Negative K6w-Ubiquitin in Lens Epithelium Using Adenoviral Vector Delays Posterior Capsular Opacification in a Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2128.
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To evaluate the effects of expression of dominant negative K6W-ubiquitin in lens epithelium using adenoviral vector on epithelial-mesenchymal transition and the formation of posterior capsular opacification (PCO) in a rabbit model.
Twenty four rabbits were divided into 4 groups randomly. Phacoemulsification was performed on both eyes. After water-tight suturation of scleral turnel incision, different quantities of recombinant dominant negative K6W-ubiquitin adenovirus and empty virus (group A, 2.82×105ifu/mL; group B, 1.13×106ifu/mL; group C, 2.26×106ifu/mL; and group D, 4.29×106ifu/mL) was diluted to 0.5mL and injected into right and left anterior chambers, respectively. Posterior capsular opacification were assessed and documented by slit lamp photography at 1, 3, 5, 7, 11 and 15 days after surgery. The degree of PCO was calculated by posterior capsule opacification manual software (POCOman). Two weeks after operation, lens capsules were dissected for immunofluorescenct detection of -SMA.
In group A, there is no statistical difference in degrees of PCO between the K6W-ubiquitin-expressed and control eyes. In group B, the degrees of PCO in K6W-ubiquitin-expressed eyes were lower than those in the control eyes at 11 and 15 days after surgery. In group C, the protective effects were observed at 5,7,11 and 15 days after surgery. In group D, the protective effects of K6W-ubiquitin expression on PCO were detected, however, severe corneal edema was also observed. The expression of -SMA in the K6W-ubiquitin group was lower than that of the control group.
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