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F. Berisha, S. Aliyeva, N. Pfeiffer, E. M. Hoffmann; Intraobserver Reproducibility of Retinal Nerve Fiber Layer Thickness Measurements Using the RTVue OCT in Normal and Glaucomatous Eyes. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2261.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the intraobserver reproducibility of Fourier-domain optical coherence tomography (FD-OCT) retinal nerve fiber layer (RNFL) thickness measurements in normal and glaucomatous eyes.
Forty eyes of 25 glaucoma patients and 25 eyes of 16 healthy subjects were included in the study. Peripapillary (RNFL) thickness measurements were performed using the RNFL thickness 3.45 circle scan mode of a FD-OCT (RTVue; Optovue Inc., Fremont, USA). Each eye underwent three RNFL measurements obtained by an experienced examiner on the same day. The short term variability for quadrant (superior, inferior, temporal and nasal) and average RNFL thickness measurements was assessed using ANOVA based intraclass correlation coefficient (ICC) and coefficient of variation (CV). Furthermore, the effect of signal strength on the variability of RNFL measurements was studied using a linear regression analysis.
The mean ICC for average RNFL measurements was 0.97 in both normal subjects and glaucoma patients. The corresponding CV values were 2.7% and 6.0% in normal and glaucomatous eyes, respectively. In control subjects the ICC values for quadrant measurements of RNFL thickness ranged between 0.93 and 0.98 in normal subjects and between 0.81 and 0.98 in glaucoma patients, indicating an excellent reproducibility of the method. The corresponding CV values ranged between 2.5% and 7.9% and between 3.3% and 9.0% in normal and glaucomatous eyes, respectively, with the inferior quadrant measurements having the highest reproducibility values and the temporal quadrant having the lowest, but still excellent reproducibility values. The CV values for signal strength were 7.1% in control subjects and 6.5% in glaucoma patients. The results showed a significant correlation between CV for temporal RNFL measurements and the signal strength variations (p<0.001).
Our results show that RNFL thickness measurements obtained with the RTVue are highly reproducible. FD-OCT seems to be a promising technology that can be used in the clinical setting both in terms of diagnosis and management of patients with glaucoma.
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