April 2009
Volume 50, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2009
Expression of Vascular Endothelial Growth Factor Receptor-3 in Laser-Induced Choroidal Neovascularization
M. Kitamura; S. He; S. Sonoda; C. Spee; S. J. Ryan; D. R. Hinton
Author Affiliations & Notes
  • M. Kitamura
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
  • S. He
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
    Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • S. Sonoda
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
  • C. Spee
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
  • S. J. Ryan
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
  • D. R. Hinton
    Ophthalmology, University of Southern California, Doheny Eye Institute, Los Angeles, California
    Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  M. Kitamura, None; S. He, None; S. Sonoda, None; C. Spee, None; S.J. Ryan, None; D.R. Hinton, None.
  • Footnotes
    Support  NIH grants EY 02061, EY 03040 & grants from RPB & the Arnold & Mabel Beckman foundation
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2344. doi:
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      M. Kitamura, S. He, S. Sonoda, C. Spee, S. J. Ryan, D. R. Hinton; Expression of Vascular Endothelial Growth Factor Receptor-3 in Laser-Induced Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2344.

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Abstract

Purpose: : To determine the expression of vascular endothelial growth factor receptor-3 (VEGFR-3) in the murine model of laser induced choroidal neovascularization (CNV) and the regulation of VEGFR-3 by inflammatory cytokines in human RPE cells in vitro.

Methods: : Cultured fetal human RPE cells were used for the study. VEGFR-3 mRNA expression was determined by RT-PCR. VEGFR-3 protein in supernatant and cell lysate was examined by western blotting. RPE cells were exposed to tert-butyl-hydroperoxide (TBH), IFN-γ and TNF- from 24~72 hours. The regulation of expression of VEGFR-3 in RPE was analyzed by western blotting. The expression of VEGFR-3 in human fetal, and adult retinal sections and murine CNV sections was evaluated by immunohistochemistry.

Results: : Human RPE cells express VEGFR-3 mRNA and protein. VEGFR-3 was detected in choroidal capillaries in fetal retina and RPE monolayer of adult retinal sections. IFN-γ increased the secretion of soluble VEGFR-3 protein in the culture medium. TBH decreased the secretion of solubleVEGFR-3 protein. Prominent immunoreactivity for VEGFR-3 was detected within laser-induced CNV membranes.

Conclusions: : Levels of soluble VEGFR-3 in cultured RPE cell media is regulated by IFN-γ and TBH. VEGFR-3 is expressed in laser induced CNV membranes in the mouse. The result suggests that VEGFR-3 may play an important role in the pathogenesis of CNV.

Keywords: retinal pigment epithelium • age-related macular degeneration • choroid: neovascularization 
© 2009, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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