April 2009
Volume 50, Issue 13
ARVO Annual Meeting Abstract  |   April 2009
Nuclear-Factor Kappa B: A Potential Role in Pterygium Formation
Author Affiliations & Notes
  • J. J. Siak
    Singapore Eye Research Institute, SingHealth Services, Singapore
  • L.-F. Seet
    Singapore Eye Research Institute, SingHealth Services, Singapore
  • R. W. Beuerman
    Singapore Eye Research Institute, SingHealth Services, Singapore
    Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health System, Singapore
  • L. Tong
    Singapore Eye Research Institute, SingHealth Services, Singapore
    Singapore National Eye Centre, SingHealth Services, Singapore
  • Footnotes
    Commercial Relationships  J.J. Siak, None; L.-F. Seet, None; R.W. Beuerman, None; L. Tong, None.
  • Footnotes
    Support  NMRC IBG, NMRC/NIG/0002/2007, BMRC 03/1/35/19/231, SHF/FG325S/2007. NMRC/TCR/002-SERI/2008 and the NUS-Exxon Mobil Fellowship for Clinician Researchers, SingHealth TDF Research Grant 2008/2009
Investigative Ophthalmology & Visual Science April 2009, Vol.50, 2620. doi:
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    • Get Citation

      J. J. Siak, L.-F. Seet, R. W. Beuerman, L. Tong; Nuclear-Factor Kappa B: A Potential Role in Pterygium Formation. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2620.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Pterygium is a common ocular surface disease manifesting as a fibrovascular conjunctival outgrowth that invades the cornea. We seek to elucidate the role of Nuclear Factor kappa B (NF-ΚB) pathway in the pathogenesis of pterygium.

Methods: : Surgically excised pterygium tissues, obtained with written informed consent in accordance with declaration of Helsinki, were studied compared to normal uninvolved conjunctiva. Immunohistochemistry, immunoblotting, and nuclear DNA binding assays against p65, Rel B, p50, p52 and c-Rel were performed to evaluate activation of various NF-ΚB members. Levels of phosphorylated inhibitor IΚB were evaluated by ELISA. Expression of NF-ΚB target genes were studied using U133A genechip microarray. Primary fibroblasts culture from pterygium explants were stimulated with increasing doses of TNF- (20ng/ml to 100ng/ml) and UVB irradiation (5mJ/cm2 to 30mJ/cm2) and immunocytochemistry, immunoblotting against above-mentioned proteins and real-time PCR against NF-ΚB target genes were studied.

Results: : p65 and p105/p50 were expressed in pterygium and conjunctival epithelium on immunohistochemistry. Increased nuclear p65 DNA binding activity indicated increased NF-ΚB activation in pterygium tissues compared to normal uninvolved conjunctiva. Levels of phosphorylated IΚB were increased in pterygium tissues using ELISA. Cultured pterygium fibroblasts showed p65 nuclear translocation after exposure to 20ng/ml TNF- and 10mJ/cm2 of UVB irradiation on immunocytochemistry. Real-time PCR showed increased transcription of IΚB, IL-6, IL-8 within 30min after UVB and TNF- stimulation, and delayed increase in MCP-1 transcription beyond 2 hours, and Rel B, MMP2, MMP3 and MMP9 beyond 12 hours after TNF- stimulation, and MMP 1 beyond 12 hours after both UVB and TNF- stimulation.

Conclusions: : The NF-ΚB pathway is activated in pterygium. Stimulation of pterygium fibroblasts by TNF- and UVB can activate NF-ΚB pathway. The control of transcription of interleukins and MMP genes may be controlled by different members of the NF-ΚB family.

Keywords: pterygium • wound healing • transcription factors 

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