Abstract
Purpose: :
This study evaluates the in vitro stability and non contamination of fortified ophthalmic antibiotic preparations of 5% ceftazidim (50 mg/ml), 5% amikacin (50 mg/ml) and 5% vancomycin (50 mg/ml) stored at -20°C for 6 months
Methods: :
Antibiotic solutions were prepared using aseptic techniques by reconstitution and dilution of commercially available solution (amikacin) or powder (ceftazidime, vancomycin) and stored at -20°C. At various storage time-points, physical and chemical stabilities were determined using pHmeter, osmometer, high-performance liquid chromatography method. The microbiological activity was assayed according to the European Pharmacopoeia.
Results: :
5% ceftazidime or amikacin in 0,9% sodium chloride and vancomycin in 5% dextrose were found to be physically, chemically and microbiologically stable at -20°C for 190 days. A time limitation of 6 months is proposed.
Conclusions: :
Fortified antibiotic ophthalmic solutions manufactured and supplied by a pharmacy can be stored in freezer at -20°C for use. In the best of our knowledge, it is the first such a long stability study for these freeze antibiotics.
Keywords: antibiotics/antifungals/antiparasitics • bacterial disease • clinical laboratory testing