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F. Mouriaux, M. Fines-Guyon, A. Chérel, A. Perdriel, F. Albessard, D. Debruyne, V. Chédru-Legros; In vitro Stability of Fortified Ophthalmic Antibiotics Stored at -20°C Over a 6 Months Period. Invest. Ophthalmol. Vis. Sci. 2009;50(13):2671.
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This study evaluates the in vitro stability and non contamination of fortified ophthalmic antibiotic preparations of 5% ceftazidim (50 mg/ml), 5% amikacin (50 mg/ml) and 5% vancomycin (50 mg/ml) stored at -20°C for 6 months
Antibiotic solutions were prepared using aseptic techniques by reconstitution and dilution of commercially available solution (amikacin) or powder (ceftazidime, vancomycin) and stored at -20°C. At various storage time-points, physical and chemical stabilities were determined using pHmeter, osmometer, high-performance liquid chromatography method. The microbiological activity was assayed according to the European Pharmacopoeia.
5% ceftazidime or amikacin in 0,9% sodium chloride and vancomycin in 5% dextrose were found to be physically, chemically and microbiologically stable at -20°C for 190 days. A time limitation of 6 months is proposed.
Fortified antibiotic ophthalmic solutions manufactured and supplied by a pharmacy can be stored in freezer at -20°C for use. In the best of our knowledge, it is the first such a long stability study for these freeze antibiotics.
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