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Y.-J. Jo, K.-H. Sonoda, Y. Oshima, T. Ishibashi; A Model of Retinal Scarring by Subretinal Injection of Peritoneal Exduative Cells(PEC). Invest. Ophthalmol. Vis. Sci. 2009;50(13):2709.
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© ARVO (1962-2015); The Authors (2016-present)
To make a model of retinal scarring by subretinal injection of periotoneal exudative cells (PEC)
PEC (0.5ul of 4x107/ml) induced by thioglycolte and PBS were injected into subretinal space of C57BL/6 and Monocyte Chemoattractant Protein 1 Knock-Out (MCP1-KO) mice. 1 week later, choroid and retinal flat mount and sections were made. The amount of glial proliferation was measured by Glial Fibrillary Acidic Protein (GFAP) staining. To investigate of effect of steroid, dexamethasone was intraperitoneally injected for 5 days. Myofibroblastic changes were detected by alpha smooth muscle actin staining.
After 1 week later, yellow-centered white prolferative tissues on retina and subretinal lesions were confirmed by fundus examination. In Hematoxylin and Eosin (H&E) staining, there were many spindle shaped cells in PEC injected area. The size of glial proliferation with GFAP staining was bigger in PEC injected group than PBS injected group (0.86um2 vs 0.27um2, p<0.01). This area was reduced by steroid treatment (2mg/kg, 100ul)(0.53um2 vs 0.21um2, p<0.01). In MCP1-KO mice, the size was reduced than control mice (0.82um2 vs 0.44um2, p<0.01). More severe myofibroblastic changes were developed in PEC than PBS injected group.
Both of exogenously injected and endogenously induced inflammatory macrophage might have an important role to induce glial proliferation in subretinal area.
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